通过在热应力下通过适应性实验室进化增强Streptococcus equinus HC5中的牛HC5产量
Rodrigo G Dias1, Yasmin N V Sabino1, Katialaine C A Domingues1
1Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Applied microbiology and biotechnology
|December 9, 2025
概括
通过增加bvcA基因表达和耐热性,适应性实验室进化显著增强了Streptococcus equinus HC5中的牛HC5产量. 这种代谢工程策略为优化细菌素产量提供了一个有希望的方法.
科学领域:
- 微生物学和生物技术
- 代谢工程是代谢工程.
- 细菌素生产生产
背景情况:
- 玻维辛HC5,一种来自Streptococcus equinusHC5的细菌素,具有抗菌性质,但产量低.
- 低产量限制了Bovicin HC5在控制病原和腐烂微生物的实际应用.
研究的目的:
- 为了研究温度对S. equinus HC5生长和牛 HC5产生的影响.
- 在热应激下使用适应性实验室进化 (ALE) 增强牛HC5产量.
- 描述由此产生的高产品种的特征.
主要方法:
- 确定了野生型S. equinus HC5菌株的最佳生长温度.
- 在高温下 (47°C和48°C) 应用ALE400代,以进行改善牛HC5生产的选择.
- 分析了bovicin HC5产量,bvcA基因表达,耐热性,生长率,生物质积累,膜组成和特定变体中的基因组突变.
主要成果:
- 对于S. equinus HC5来说,最佳的生长温度是42°C;生长停止在49°C以上.
- 两种ALE衍生的变体显示,牛HC5产量增加了140%.
- 与野生类型相比,产量最高的变种 (S. equinus HC5 40048) 在48°C的温度下表现出增加的bvcA表达,增强的耐热性,更高的生长率 (1.33h-1),以及更大的生物质 (OD600nm = 4.03) (0.98h-1,OD600nm = 1.96).
结论:
- 适应性实验室进化是一种有效的策略,可以增强S. equinus HC5.5中的牛HC5产量.
- 由ALE产生的变异体表现出更好的耐热性和代谢特性,与调节蛋白质修饰,转录和运输的基因突变有关.
- 这项研究为优化细菌生物合成和开发S. equinus HC5作为微生物生产平台提供了宝贵的见解.
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