转录因子PcDfd调节了PcCCE5的表达,该因子与Panonychus citri (McGregor) 中的螺旋二烯耐药性有关
Qi-Qi Yang1, Si-Chen Li1, Qi Pan1
1Citrus Research Institute, Southwest University, National Citrus Engineering Research Center, Chongqing, China.
Pest management science
|December 10, 2025
概括
转录因子PcDfd调节碳素/胆化酶 (CCE) 基因PcCCE5,增强了Panonychus citri.中的螺旋二烯耐药性. 这项研究揭示了PcDfdd.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 炭基/胆酶 (CCE) 对于代谢内源和外源化合物至关重要.
- CCE基因PcCCE5与Panonychus citri.中的螺旋双烯耐药性有关.
- 对于 PcCCE5 在抗素耐药性方面的调控机制在很大程度上是未知的.
研究的目的:
- 阐明了Panonychus citri.中PcCCE5基因的调节机制.
- 为了确定负责调节PcCCE5表达的转录因子.
- 了解这种调节途径在螺旋二烯耐药性中的作用.
主要方法:
- 在PcCCE5促进器区域中识别cis作用元素.
- 双露西法酶记者测定用于评估转录活性.
- 酵母单杂交 (Y1H) 和电泳性移动性转移试验 (EMSA) 来确认蛋白质-DNA相互作用.
主要成果:
- PcCCE5的cis作用元件定位在 -674 和 -483 bp之间.
- 转录因子PcDfd显著增强了PcCCE5的转录.
- PcDfd直接与PcCCE5促进体结合,在耐药菌株和暴露于螺旋二克洛芬时的表达增加.
- 沉默PcDfd降低了PcCCE5的表达,并增加了对螺旋二芬的敏感性.
结论:
- 转录因子PcDfd通过与"TAATTGTT"基因结合,对PcCCE5的表达进行上调.
- 这种PcDfd-PcCCE5相互作用是赋予Panonychus citri.中螺旋二烯耐药性的关键机制.
- 这是第一份报告,证明了PcDfd在抗素耐药性中的作用.
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