牙周炎的生物标志物通过热溶解-气相色谱-质谱分析分析
Qin Liang1, Yanling Zhang2, Xiaoli Zhang3
1Department of Preventive Dentistry, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & NHC Key Laboratory of Digital Stomatology & NMPA Key Laboratory for Dental Materials, Beijing, China.
这项研究确定了环松作为一种潜在的牙周炎诊断生物标志物,使用呼吸和唾液中发现的挥发性有机化合物. 这些发现表明,牙周病的新诊断方法.
科学领域:
- 口腔健康 口腔健康
- 生物标志物发现发现
- 代谢学 代谢学 代谢学
背景情况:
- 牙周炎是一个重要的全球公共卫生问题.
- 唾液挥发性代谢物显示为牙周病的诊断生物标志物具有前途.
- 关于在牙周炎患者中收集和识别这些代谢物的研究有限.
研究的目的:
- 在牙周炎患者中探索收集和识别唾液挥发性代谢物的方法.
- 研究这些代谢物的潜力,作为诊断牙周病的生物标志物.
主要方法:
- 收集了来自115名牙周炎患者和35名健康患者的口腔呼气和唾液样本.
- 采用热溶解气色谱-质谱法 (TD-GC-MS) 进行呼吸中的挥发性有机化合物 (VOC) 分析.
- 采用固态微提取气体染色体质谱法 (SPME-GC-MS) 进行唾液分析,并采用液体染色体质谱法 (LC-MS) 进行代谢途径调查.
主要成果:
- 在使用TD-GC-MS检测的78种化合物中确定了14种不同的VOC.
- 开发了一种使用环素, styrene 和乙醇的诊断模型,其 AUC 为 0.8237.7.
- 在唾液中检测到这些代谢物,在牙周炎患者 (P <0.05) 中,循环松的表达更高.
结论:
- 开发了一种新的TD-GC-MS方法用于唾液挥发性代谢物分析,显示了牙周炎诊断的潜力.
- 确定了环松作为牙周炎的潜在生物标志物.
- 突出了卡普罗拉克塔姆降解途径在牙周炎中口腔微生物群失生症中的潜在作用.
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