抗体研究中的综合性和新兴模型:综合性审查
Jagadeeswara Reddy Devasani1, Girijasankar Guntuku1, Prathyusha Sarabu1
1Pharmaceutical Biotechnology Division, A.U. College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh 530003, India.
Antibody therapeutics
|December 10, 2025
概括
本综述探讨了用于抗体研究的体内,体外和体模型. 整合这些方法可以提高抗体的发现和各种疾病的治疗方法的开发.
科学领域:
- 生物技术是生物技术.
- 免疫学 免疫学 免疫学
- 计算生物学 计算生物学
背景情况:
- 抗体研究利用了体内,体外和体模型,每个模型都有独特的优点和缺点.
- 在体内模型 (动物,人性化小鼠) 提供生理洞察力,但面临道德和翻译障碍.
- 试验室方法 (混合瘤,菌体显示) 允许有效的查,但缺乏现实世界的适用性.
研究的目的:
- 在抗体研究中批判性地评估当前的体内,体外和体内模型.
- 强调这些多样化的模型的整合,以克服现有的局限性.
- 突出突出的新兴技术革命抗体的发现和开发.
主要方法:
- 在体内 (动物,人性化小鼠) 模型的审查和批判性评估.
- 在体外技术的分析 (混合瘤,菌体显示,B细胞培养).
- 评估in silico方法 (计算生物学,机器学习).
- 探索新兴技术 (CRISPR,单细胞测序,微流体学,器官在芯片上).
主要成果:
- 在体内模型提供了疗效和药理动力学数据,但存在伦理/翻译问题.
- 试管体内方法在选/优化方面表现出色,但缺乏生理相关性.
- 在 silico 方法加速设计和预测,提高成本和可扩展性.
- 新兴技术有望显著推进抗体发现.
结论:
- 整合体内,体外和内模型对于克服可再生性和免疫性预测等挑战至关重要.
- 利用这些综合模型的多学科方法将推动下一代抗体治疗的发展.
- 进展有望改善癌症,自身免疫性疾病和传染病的治疗方法.
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