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辅酶通过调节染色质可访问性和植物再生过程,影响基因编辑效率

Zhaoyuan Lian^1,2, Tao Jiang², Yufei Liang¹

¹College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.

Horticulture research

|December 10, 2025

概括

在植物组织培养过程中增加auxin水平通过提高DNA可访问性和允许更多时间进行编辑来提高CRISPR基因编辑效率. 一种双相培养方法可以提高编辑植物的再生.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

对于作物遗传增强,CRISPR/Cas9技术至关重要.
优化基因编辑效率是一个关键的研究目标.

研究的目的:

研究外源性奥克辛对植物CRISPR/Cas9基因编辑效率的影响.
阐明奥克辛对基因编辑的影响背后的机制.
开发一种改进的培养策略,用于植物基因编辑.

主要方法:

在体外组织培养中,使用不同度的辅酶.
用于基因表达分析的RNA测序 (RNA-Seq).
用测序 (ATAC-Seq) 来进行染色核和转移酶可访问的染色质的染色,用于染色质结构分析.
制定和验证一个两阶段的文化战略.

主要成果:

素水平升高显著提高了基因编辑效率和编辑的T₀植物的频率.
更高的辅酶促进了的生长,但延迟了发芽的开始和略微减少了再生.
辅助治疗导致了更轻松的染色质结构,增加了Cas9.9的DNA可访问性.
一个两阶段的培养策略成功地平衡了增强的编辑与拍摄再生.

结论:

外源性辅酶通过改变染色质可访问性和提供延长编辑时间,积极影响CRISPR/Cas9基因编辑效率.
这些发现为奥克辛在基因编辑中的作用提供了新的见解,并为未来的研究提供了理论基础.
开发的双相培养方法可以加速在各种植物物种中应用基因编辑技术.

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