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发现和血清学验证DAMP衍生的B细胞表位作为糖尿病病的诊断生物标志物

Chengyuan Yu¹, Yishi Dong¹, Fuhua Zhong²

¹Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatrics, Department of Geriatrics, Shenzhen People's Hospital (The First Affiliated Hospital, Southern University of Science and Technology, The Second Clinical Medical College, Jinan University), Shenzhen, China.

Frontiers in endocrinology

|December 10, 2025

概括

研究人员从与损伤相关的分子模式 (DAMPs) 中确定了新型标记物,用于诊断糖尿病病 (DN). 这些DAMP衍生具有高免疫性和特异性,为早期DN检测铺平了道路.

科学领域:

* 免疫学免疫学
* 分子生物学 * 分子生物学
* 腎病學腎病學

背景情况:

* 糖尿病病 (DN) 是一种严重的糖尿病并发症,也是末期病的主要原因.
*由损伤相关分子模式 (DAMP) 驱动的慢性炎症,如HMGB1,S100A8和S100A9,与DN有很强的联系.
* 确定特定的生物标志物对于早期DN诊断和管理至关重要.

研究的目的:

* 识别和验证来自HMGB1,S100A8和S100A9.9的保存的B细胞表位.
* 开发基于的新型血清标记物,用于早期诊断糖尿病病.
* 评估患者血清中这些表位的诊断潜力.

主要方法:

*从NCBI RefSeq.中检索HMGB1,S100A8和S100A9的正规序列.
*使用生物信息学工具 (BepiPred,ABCpred,ElliPro) 预测和评估线性和构形B细胞表位.
*使用DN患者和健康对照的间接ELISA对血清进行候选的合成和验证.

主要成果:

*从HMGB1,S100A8和S100A9.9中确定了三种免疫性和进化保守的线性B细胞表位.
*与对照组相比,ELISA在DN患者血清中显示了对这些的IgG反应显著升高 (p <0.01).
*来自HMGB1的表现出最强大和最具特异性的免疫反活性,表明强大的诊断潜力.

结论:

*新型DAMP衍生的B细胞表位,具有糖尿病病的显著诊断潜力,已成功识别和验证.
*开发的具有较高的免疫性,特异性,在血清学测试中表现一致.
*这些发现支持开发用于早期DN检测的非侵入性,基于的诊断工具.

关键词:

B细胞的表观特征这就是HMGB1的特点.在S100A8A8中使用.在S100A9A9中使用.糖尿病性脏病糖尿病脏病基于的ELISA测试方法基于的诊断方法.血清学的生物标志物

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