相关实验视频
Updated: Jan 9, 2026

10:40
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
Published on: April 25, 2022
2.8K
与迁移体相关的长非编码RNA编排免疫微环境,并作为清细胞细胞癌的新奇预后模型
Junming Huang1, Juezhuo Huang2, Jueling Wei1
1Department of Urology, Guangxi Medical University Cancer Hospital, Nanning, China.
Translational andrology and urology
|December 10, 2025
概括
这项研究确定了五种与迁移体相关的长非编码RNA (MRL),可以预测清细胞细胞癌 (ccRCC) 患者的存活率. 这种MRL签名改善了风险分层,并表明了ccRCC的潜在治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 清细胞细胞癌 (ccRCC) 是最常见的癌亚型.
- 在ccRCC中,与迁移体相关的长非编码RNA (MRL) 的预后意义尚不清楚.
- 识别新的预后标志物对于改善ccRCC患者的治疗结果至关重要.
研究的目的:
- 系统地确定与ccRCC预后相关的MRL.
- 为ccRCC风险分层开发一个强大的预后模型.
- 探索基于MRL和相关途径的潜在治疗策略.
主要方法:
- 分析了TCGA ccRCC患者的转录组数据.
- 使用LASSO回归来建立基于MRL表达的预后特征.
- 单细胞RNA测序 (scRNA-seq) 用于分析细胞异质性.
- 在临床样本中,RT-qPCR验证了关键的lncRNAs.
主要成果:
- 一个五个MRL预后签名 (EMX2OS,AC106897.1,AC087645.2,AC121338.2,C5orf66) 的建立.
- 签名独立预测了ccRCC患者的整体存活率 (OS).
- 免疫景观分析揭示了高风险和低风险群体之间的独特免疫特征.
- scRNA-seq确定了17个细胞亚群,并涉及到VEGF信号传递在迁移体功能中.
结论:
- 五个MRL签名为ccRCC提供了有价值的预后见解.
- 这种签名可以帮助临床风险分层和治疗决策.
- VEGF信号调制为ccRCC提供了潜在的治疗途径,针对迁移和迁移体功能.
相关概念视频
lncRNA - Long Non-coding RNAs
9.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.7K
MicroRNAs
3.7K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.7K
MicroRNAs
23.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.8K
Non-LTR Retrotransposons
13.1K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
13.1K
siRNA - Small Interfering RNAs
18.3K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.3K
Abnormal Proliferation
5.1K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K