相关实验视频
Updated: Jan 9, 2026
13:04
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Published on: March 1, 2019
9.3K
繁荣的长非编码RNA表达可能雕刻Igh locus拓
Ellen B Drake1, Sarah Naiyer1, Xinyan Qu1
1Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago, IL, United States.
Frontiers in immunology
|December 10, 2025
概括
长非编码RNAs (lncRNAs) 和凝聚素介导的循环挤出是免疫球蛋白重链 (Igh) 基因重组的关键. 这项研究揭示了Igh位点的lncRNAs影响V(D) J重组和基因使用.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 多种免疫球蛋白重链 (Igh) 谱系对于适应性免疫是必不可少的,它们依靠V(D) J重组用于B细胞受体表达和抗体分泌.
- 高位缩,由凝聚素介导的循环挤出促进,使远距离V(D) J基因段更接近重组中心,但控制差异V(D) J基因使用的机制仍然不清楚.
研究的目的:
- 研究长非编码RNAs (lncRNAs) 在调节Igh locus拓和V(D) J重组中的作用.
- 阐明lncRNAs,增强剂和染色质循环之间的相互作用,以确定V(D) J基因段的可访问性和使用.
主要方法:
- 在免疫球蛋白重链 (Igh) 位点和其他抗原受体 (AgR) 位点对lncRNA表达的分析.
- 对 lncRNA 位置与 Igh 增强剂和色素循环的相关性分析.
- 整合lncRNA功能与凝聚力介导的循环挤出模型.
主要成果:
- 在Igh位点和其他V(D) J重组位点观察到异常高的lncRNAs表达.
- 多外显性lncRNAs,Igh增强剂和染色质循环点的基因组位置之间存在强烈的相关性.
- 这些发现表明,lncRNAs和结构元素在塑造Igh locus架构方面发挥着协调作用.
结论:
- lncRNAs在Igh位点显著表达,并且与调节元素和染色质环空间相关.
- 一个涉及lncRNAs和循环挤出的综合模型被提议用于解释Igh locus拓和V(D) J重组动态.
- 这项研究突出了V(D) J重组的新型调节机制,影响B细胞发育和幽默免疫力.
相关概念视频
03:17Inheritance of Chromatin Structures
7.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.2K
02:39lncRNA - Long Non-coding RNAs
9.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.7K
02:39lncRNA - Long Non-coding RNAs
3.5K
3.5K
02:32Exon Recombination
4.0K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
4.0K
02:24Regulation of Expression Occurs at Multiple Steps
25.6K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
25.6K
02:24Regulation of Expression Occurs at Multiple Steps
3.8K
3.8K