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Updated: Jan 9, 2026

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

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Published on: September 2, 2013

一种精简的技术,用于深入 ubiquitomics 分析甲固定的嵌组织

Shifang Liang^1,2, Bo Zheng³, Dongying Huang¹

¹State Key Laboratory of Medical Proteomics, National Center for Protein Sciences (Beijing), Beijing Proteome Research Center, Academy of Military Medical Sciences, Beijing 102206, China. yingwantao@ncpsb.org.cn.

Analytical methods : advancing methods and applications

|December 10, 2025

概括

固定在甲中的胺嵌入式组织对无处不在学有价值. 这项研究提出了一种优化的方法,可以从这些组织中提取GlyGly蛋白质,从而使全面的无处不在研究成为可能.

科学领域:

生物化学生物化学
蛋白质组学是指蛋白质组学.
分子生物学分子生物学

背景情况:

与新鲜冷 (FrFr) 组织相比,在长期储存和样本可用性方面,固定在形式氨基嵌 (FFPE) 组织具有优势.
乌比基因化是一种关键的翻译后修饰,它调节了许多细胞过程,但其在FFPE组织中的研究具有挑战性.
目前的无处不在学研究主要依赖于新鲜冷的组织,限制了FFPE样本的潜力.

研究的目的:

开发和优化一种精简的技术,从FFPE组织中提取GlyGly蛋白.
为了使用FFPE样本进行全面的无处不在研究.
评估储存时间对FFPE组织中无处不在和蛋白质组学信息的影响.

主要方法:

针对FFPE组织提取的优化溶解缓冲组合.
精制组织部分的加热持续时间,以提高蛋白质的可访问性.
改善了蛋白质消化策略,以实现高效的GlyGly回收.
开发的方法应用于肝细胞癌 (HCC) FFPE样本.

主要成果:

从HCC FFPE组织段中成功识别出7000-10,000个GlyGly位点.
证明存储时间对7年内无处不在数据稳定性的影响最小.
显示,蛋白质组学信息在FFPE组织中保持稳定超过10年.

结论:

优化的技术使使用FFPE组织进行全面的无处不在学研究成为可能.
在无处不在学研究中,FFPE织物是FrFr织物的有价值且具有成本效益的替代品.
这些发现支持扩大FFPE档案的使用范围,用于生物标志物发现和疾病机制探索.

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