在酶发酵过程中细菌群体的结构和功能变化的表征
Zhugui Wen1, Xinyu Liu2, Simiao Ma2
1School of Wetlands, Yancheng Teachers University, Yancheng, 224007, China. wenzhugui@163.com.
Current microbiology
|December 10, 2025
概括
农业酶在液体和堆肥发酵中对土壤微生物群落的影响不同. 虽然酶在液体发酵中减少了物种,但堆肥显示出多样性增加和统一的类型丰富,为农业应用提供了洞察力.
科学领域:
- 农业科学 农业科学
- 微生物学 微生物学
- 环境科学 环境科学
背景情况:
- 土壤健康对农业至关重要,微生物群落发挥着至关重要的作用.
- 酶应用正在成为管理土壤问题的战略.
- 由于环境影响,在发酵过程中对微生物群落的表征是复杂的.
研究的目的:
- 为了分析细菌社区结构和功能特征在堆肥发酵过程中和没有酶添加.
- 为了比较外源酶对液体发酵中的微生物群落的影响与堆肥.
主要方法:
- 使用16SrRNA高通量测序来分析细菌社区结构.
- 基因和基因组的京都百科全书 (KEGG) 数据库被用来识别微生物的功能特征.
- 在中后阶段分析了堆肥和液体发酵样本.
主要成果:
- 外源酶减少了液体发酵中的微生物物种,导致蛋白质细菌占主导地位 (70%).
- 在堆肥中,细菌的丰富性随着发酵时间的增加而增加,显示出统一的类型分布.
- 确定的主要代谢途径与碳水化合物,氨基酸和辅助因子代谢有关.
结论:
- 外源酶补充剂在液体和堆肥发酵过程中显著改变了微生物群落的继承和代谢功能.
- 酶的应用为改善传统的堆肥和农业实践提供了理论基础.
- 了解微生物对酶的反应是可持续农业的关键.
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