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对病毒广泛中和抗体针对OPG153的抗原无定性鉴定

Ida Paciello¹, Emily J Rundlet², Ling Zhou²

¹Monoclonal Antibody Discovery (MAD) Laboratory, Fondazione Toscana Life Sciences, Siena 53100, Italy.

Science translational medicine

|December 10, 2025

概括

研究人员确定了一种关键的水病毒 (MPXV) 蛋白质OPG153,作为广泛中和抗体的目标. 这一发现支持OPG153作为MPXV疫苗和抗体疗法的有希望的抗原.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
结构生物学结构生物学

背景情况:

经常出现的mopox疫情需要开发有效的医疗干预措施.
病毒 (MPXV) 构成了全球健康的重大威胁.

研究的目的:

确定针对MPXV的广泛中和抗体的目标.
评估已识别的标作为疫苗抗原和治疗标的潜力.

主要方法:

对MPXV中和单克隆抗体的抗原不可知识别.
基于AlphaFold 3的预测建模.
低温电子显微镜. 电子显微镜.

主要成果:

由正病毒基因 (OPG) 153 (MPXV A28) 编码的蛋白质被确定为广泛中和抗体的目标.
针对OPG153的抗体中和了MPXV的Ib和IIb类,疫苗病毒 (VACV),并与牛和天花病毒的ortolog交叉反应.
在小鼠中,用MPXV OPG153进行免疫接种诱导了针对MPXV和VACV的强有力的中和抗体反应.

结论:

MPXV OPG153是一种有前途的疫苗抗原.
MPXV OPG153是针对mopox病毒和相关的orthopoxvirus的预防性和治疗性抗体的有效标.

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