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Updated: Jun 30, 2026

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

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使用彗星试验评估的双链精子DNA碎片化与反复出现的怀孕流产有关

Andrew Drakeley1, Rebecca L Lunt2, Emily Newton2

  • 1Hewitt Fertility Centre, Liverpool Women's NHS Foundation Trust, Liverpool, UK.; University of Liverpool, Liverpool, UK..

Reproductive biomedicine online
|December 10, 2025
PubMed
概括

参观反复流产诊所的男性显示高水平的双链精子DNA碎片化 (dsSDF). 这凸显了调查重复流产的男性因素的关键需要.

关键词:
彗星测量试验方法在DNA碎片化过程中,DNA碎片化.双链DNA断裂是因为双链DNA断裂.经常发生的流产.

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DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...