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麦蛋白缩物的酶挤出加工:结构,功能和营养特性
Tizazu Yirga Bereka1, Xing Zhou2, Ren Wang2
1School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Department of Postharvest Management, Jimma University College of Agriculture and Veterinary Medicine, 307 Jimma, Ethiopia.
Food chemistry
|December 10, 2025
概括
酵素挤出水解有效地产生具有增强功能的麦蛋白缩物 (OPC). 这种可扩展的方法为各种食品应用量身定制蛋白质结构和特性.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生化学
- 材料科学 是一种材料科学.
背景情况:
- 麦蛋白缩剂 (OPC) 是从麦粉中提取的有价值的成分.
- 传统的OPC生产方法往往在效率和功能性质方面存在局限性.
研究的目的:
- 使用酶式挤出水解来生产麦蛋白缩物 (OPC).
- 评估生产的OPCs的组成,结构和功能.
- 调查乙醇脱脂对OPC特性的影响.
主要方法:
- 前凝化全麦粉经过酶激挤出水解,使用不同的α-氨酶处理.
- 使用FTIR,CD和SEM进行了结构性表征.
- 评估了功能性质,包括可溶性,持水能力,持油能力和乳化活性/稳定性.
- 乙醇脱脂被应用于修改的OPCs.
主要成果:
- 通过高热α-氨酶 (Thr-NDFOPC) 生产的OPC显示出最高的蛋白质含量 (58.78%).
- 结构分析显示了重要的蛋白质网络破坏和展开,特别是在Thr-NDFOPC中.
- 脱脂增加了蛋白质度 (高达74.88%),并增强了水化和乳化能力.
- 溶解度依赖于pH值,在性条件下得到改善.
结论:
- 酶挤出水解是一种可行的和可扩展的技术,用于生产具有可调节性质的OPC.
- 该方法允许修改麦蛋白结构,从而改善食品应用的功能属性.
- 进一步优化处理参数可以产生具有特定所需特征的OPC.
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