酸光转化维持了Nrf2介导的抗氧化防御,防止UVA损伤
Apeksha Vikram1, Sunil Kumar Patel1, Diksha Pathania1
1Photobiology Laboratory, Food, Drug & Chemical Environment and Systems Toxicology (FEST) Division, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Qaisar Bagh, Lucknow, Uttar Pradesh-226001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh 201002, India.
酸 (FA) 在紫外线下转化为咖啡酸 (CA),保持其抗氧化和光保护功效. 这种稳定的转化使得FA成为防晒产品中一个有前途的成分.
科学领域:
- 皮肤病学和光生物学
- 生物化学和分子生物学
背景情况:
- 长期暴露于UVA会通过氧化应激和DNA损伤导致皮肤衰老.
- 自然抗氧化剂往往缺乏光稳定性,在紫外线下可能成为亲氧化剂.
研究的目的:
- 评估酸 (FA) 的光稳定性和对紫外线辐射的保护作用.
- 为了调查FA的紫外线诱导的转化产品是否保持保护活性.
主要方法:
- 分析了使用UV-Vis和LC-QTOF-MS的FA光转换.
- 在暴露于UVA的HaCaT细胞 (ROS,线粒体功能,DNA损伤) 中评估光保护.
- 使用RT-PCR和in-silico对接 (FA与Keap1) 进行分子分析.
主要成果:
- 在UVA下,FA转化为咖啡酸 (CA),两者都保持了抗氧化能力.
- FA 证明了ROS的减少,线粒体的稳定,并将DNA损伤降到最低.
- 分子分析证实了Nrf2-Keap1通路的激活,并减少了氧化/炎症标记.
结论:
- 酸通过转化为咖啡酸表现出独特的光稳定性,保持有效性.
- FA的双重作用 (ROS清除,Nrf2通路激活) 提供了显著的光保护.
- 由于其稳定,保护性的光转化,FA是防晒配方中一个有希望的,安全的生物活性成分.
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