相关实验视频

Updated: Jan 9, 2026

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Author Spotlight: A Cost-Effective Genomic Workflow for Advancing Rabies Control in Resource-Limited Settings

Published on: August 18, 2023

纳米孔测序揭示了与不同SARS-CoV-2变种相关的独特鼻微生物群落

Isnaini Zakiyyah Asyifa¹, Zahra Noviana², Alvira Rifdah Sativa²

¹Research Center for Applied Microbiology, National Research and Innovation Agency (BRIN), Jl.Raya Bogor KM46, Cibinong, 16911, Indonesia; Biomedical Masters Program, Faculty of Medicine, University of Indonesia, Jl. Salemba Raya No. 6, Jakarta, 10430, Indonesia.

Microbial pathogenesis

|December 10, 2025

概括

在COVID-19患者中,SARS-CoV-2变种改变了呼吸道微生物群. 细菌群落在阿尔法,三角洲和印度尼西亚等变种中显著不同,影响了感染动态.

科学领域:

微生物学微生物学
病毒学病毒学
基因组学就是基因组学.

背景情况:

SARS-CoV-2 变种可能会增加 COVID-19 的严重程度.
呼吸道微生物群在抵抗感染的免疫防御中起作用.
SARS-CoV-2 病原可能会破坏呼吸道微生物社区平衡.

研究的目的:

为了研究不同SARS-CoV-2变异的COVID-19患者中的细菌社区概况.
了解特定的SARS-CoV-2变种如何影响呼吸道微生物群组成.

主要方法:

通过使用牛津纳米孔技术 (ONT) 来从鼻腔/耳鼻口抽样中对SARS-CoV-2的全基因组测序.
16S rRNA基因扩增序列测序用于分析细菌社区多样性.
鉴定SARS-CoV-2变种,包括阿尔法,三角洲和印尼.

主要成果:

已经确定了6种SARS-CoV-2变种,主要是Delta,Alpha和印度尼西亚.
阿尔法和三角形变体样本显示了链球菌,Prevotella,Corynebacterium和Veillonella的优势.
印度尼西亚变种样本的特征是葡萄球菌,沙门氏菌,菌和Corynebacterium.
普雷沃特拉是总体上占主导地位的细菌属 (16.2%).

结论:

基于SARS-CoV-2变种的COVID-19患者的鼻腔/耳鼻的细菌群体中存在显著的多样性.
特定的SARS-CoV-2变种与呼吸道中不同的细菌社区结构有关.

关键词:

在 COVID-19 疫情中,微生物社区的微生物社区.一个纳米孔.这就是SARS-CoV-2病毒.测序测序是指测序的时间.变种变种变种变种

更多相关视频

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Published on: June 30, 2023

相关概念视频

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Viral Mutations

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...