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人工卵子通过鼠标子宫内膜层细胞和骨髓衍生中酶体干细胞的哈普洛化产生人工卵子

Shelun Tsai¹, Philip Xie², Stephanie Cheung²

¹The Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medical College, New York, New York; Pacific Northwest Fertility and IVF Specialists, Bellevue, Washington.

|December 10, 2025

概括

子宫内膜层细胞 (EmSCs) 和骨髓衍生中介质干细胞 (BM-MSCs) 可用于体细胞平分化,产生可活的后代. 这项研究探讨了它们作为生育治疗的替代捐赠核来源的潜力.

科学领域:

生殖生物学生殖生物学
干细胞生物学干细胞生物学
遗传学是一个遗传学.

背景情况:

体细胞平分化是一种使用捐赠细胞核创建功能性卵子细胞的技术.
识别替代性,高效的供体细胞来源对于推进辅助生殖技术至关重要.

研究的目的:

评估使用子宫内膜层细胞 (EmSCs) 和骨髓衍生介质干细胞 (BM-MSCs) 作为体细胞平分化供体细胞核的可行性.
评估从这些细胞中获得的胚胎的发育潜力和形态动力学.

主要方法:

鼠标卵细胞被去核,并用来自累积细胞 (CCs),EmSCs或BM-MSCs的细胞核进行重建.
卵细胞经历了体细胞核转移 (SCNT),随后是受精和体外培养.
评估了胚胎发育,形态动力学和后代的生存能力.

主要成果:

与EmSCs和BM-MSCs的SCNT导致了与CCs可比的融合速率.
来自EmSC和BM-MSC的胚胎显示出比CCs更高的囊胚发育率.
从EmSC和BM-MSC衍生的卵细胞成功产生了后代,证明了双亲遗传和生殖能力.

结论:

EmSCs和BM-MSCs是体细胞平化可行的供体细胞来源.
这种方法可以产生能够产生健康后代的卵细胞.
使用这些细胞的新产子生成为卵细胞因子不孕症提供了潜在的生育治疗方法.

关键词:

实体细胞的核转移转移人工卵子细胞的人工卵子细胞介质变化 (meiosis) 是一种变质的过程.新产生的新产物.身体细胞的单体化和单体化.

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