使用全基因组测序,在日本从患病的黄尾菌中分离出来的乳球球菌的表征
Manao Ozawa1, Motoshi Kawano1, Hitoshi Abo1
1National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries.
The Journal of veterinary medical science
|December 10, 2025
概括
黄尾菌中乳球菌的全基因组测序揭示了常见的血清型和高林氏素耐药性. 与其他鱼类菌株的遗传差异表明,对于疫苗的疗效要谨慎,强调对抗菌素敏感性测试.
科学领域:
- 水生微生物学 水生微生物学
- 鱼类疾病诊断 诊断 鱼类疾病诊断
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 乳球菌病是由乳球菌引起的,影响鱼类的健康.
- 对于日本黄尾乳球菌株的遗传数据有限.
研究的目的:
- 调查黄尾乳球菌中的毒性和抗菌素耐药性基因.
- 使用全基因组测序 (WGS) 确定分离物之间的遗传关系.
主要方法:
- 从患病的黄尾菌中分离出40种乳球菌的全基因组测序 (WGS).
- 对毒性基因,抗菌素耐药性基因和家族遗传关系的分析.
- 分离物的血清型定.
主要成果:
- 确定了血清型II (82.5%) 和血清型I (17.5%);没有血清型III.
- 检测到对林氏素 (85.0%) 和红色素 (12.5%) 的高耐药性.
- 红素耐药性与等离子体上的 erm(B) 基因有关.
结论:
- 黄尾乳球菌株与其他鱼类相比,具有独特的遗传特征.
- 遗传变异需要对当前疫苗疗效保持谨慎.
- 对抗菌素敏感性测试对于有效治疗乳球菌病至关重要.
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