新型转录因子TrTRC-1控制氨基酸生物合成,以调节Trichoderma reesei中的细胞酶和西兰酶生产
Xiaozan Dai1, Guanchen Liu1, Quan Zhang2
1MOE Key Laboratory of Bio-Intelligent Manufacturing, Frontiers Science Centre for Smart Materials Oriented Chemical Engineering, School of Bioengineering, Dalian University of Technology, Dalian 116024, China.
Journal of agricultural and food chemistry
|December 10, 2025
概括
研究人员确定了TrTRC-1,Trichoderma reesei中的转录因子,它调节了酶生产和新陈代谢. 删除TrTRC-1可以提高工业酶产量,改善真菌生长,为微生物菌株的发展提供了一个新的战略.
科学领域:
- 工业生物技术 工业生物技术
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- 三真菌是工业酶生产的关键真菌.
- 了解与新陈代谢相关的酶生产调节对于优化产量至关重要.
研究的目的:
- 为了描述T. reesei.中的新型C2H2指转录因子TrTRC-1的特征.
- 研究TrTRC-1在协调酶生物合成与氨基酸代谢和细胞生长中的作用.
主要方法:
- 在T. reesei Rut C30中删除TrTRC-1的基因.
- 酶活性测定 (细胞酶,西兰酶,FPase).
- 转录组分析以评估基因表达变化.
- 生物反应器培养用于工业酶生产的评估.
主要成果:
- 删除TrTRC-1显著增强细胞酶,西兰酶和细胞外蛋白质的产生 (9.8-32.4%).
- 删除TrTRC-1还刺激了菌根细胞的生长和结合.
- 转录组数据揭示了参与初级代谢,氨基酸生物合成和碳水化合物活性酶的基因的改变表达.
- T. reesei ΔTrTRC-1 实现了高的 FPase 活性 (17.35 IU/mL) 和高效的红细胞纤维素生物质糖化.
结论:
- 在T. reesei中,TrTRC-1充当多功能调节剂,平衡酶生产与细胞代谢.
- 这项研究促进了对T. reesei. 的监管网络的理解.
- 准TrTRC-1为工程微生物菌株提供了一种战略方法,用于增强工业酶生产.
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