Jove
Visualize
联系我们

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

TCP3 is a substrate of the COP1/SPA ubiquitin ligase to regulate anthocyanin accumulation and flowering time in <i>Arabidopsis</i>.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Water wisteria genome reveals environmental adaptation and heterophylly regulation in amphibious plants.

Plant, cell & environment·2024
Same author

Sugar cravings during stress: Abscisic acid-mediated starch degradation promotes plant drought tolerance.

Plant physiology·2022
Same author

What plants do in the shadows: Gene transcription precedes histone methylation during shade responses.

Plant physiology·2022
Same author

B-BOXing against UV rays: The BBX11-HY5 feedback loop regulates plant ultraviolet B tolerance.

Plant physiology·2022
Same author

Dephosphorylate to close: type 2C protein phosphatases regulate stomatal aperture.

Plant physiology·2022
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jan 9, 2026

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
07:55

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Published on: November 20, 2017

14.7K

对植物蛋白与蛋白的相互作用进行定量分离火虫光酶补充试验 (SplitLUC).

Qianwei Liu1, Rainer Kembügler2, Francesc Felipe2

  • 1Joseph Gottlieb Kölreuter Institute for Plant Sciences (JKIP), Karlsruhe Institute of Technology (KIT), 76131, Karlsruhe, Germany.

Protoplasma
|December 10, 2025
PubMed
概括

这项研究提出了一种强大的分裂火虫光酶补充试验 (SplitLUC),用于检测植物蛋白与植物蛋白相互作用 (PPI). 基于光的规范化确保了对这些基本分子相互作用的可靠量化.

关键词:
这就是为什么CCD是CCD.冷却的电荷合装置.黑夜的阴影 黑夜的阴影蛋白质蛋白质相互作用分裂的火虫光酶补充试验.分成LUCLUC 分成

更多相关视频

Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay
05:28

Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay

Published on: September 10, 2020

2.7K
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
12:55

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

Published on: June 1, 2014

24.3K

相关实验视频

Last Updated: Jan 9, 2026

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
07:55

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Published on: November 20, 2017

14.7K
Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay
05:28

Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay

Published on: September 10, 2020

2.7K
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
12:55

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

Published on: June 1, 2014

24.3K

科学领域:

  • 植物分子生物学 植物分子生物学
  • 生物化学 生物化学
  • 遗传学 遗传学 是一个

背景情况:

  • 了解蛋白质与蛋白质相互作用 (PPI) 对植物发育和应激反应至关重要.
  • 分裂火虫光酶补充试验 (SplitLUC) 是研究PPI的一个强大的工具.

研究的目的:

  • 证明和验证SplitLUC测定用于检测和量化植物中的PPI.
  • 使用SplitLUC测定来研究DDA1和PYL8之间的相互作用.

主要方法:

  • 使用冷却的基于CCD的植物成像系统和微板阅读器用于SplitLUC.
  • 与DDA1-nLUC和cLUC-PYL8结构一起透的Agrobacterium菌株.
  • 针对TagRFP光的规范发光信号,以准确量化.

主要成果:

  • 一个强大的发光信号证实了DDA1和PYL8.8之间的相互作用.
  • 对照实验显示没有可检测的发光,证实了测试特异性.
  • 基于光的正常化有效降低了发光信号的变化.

结论:

  • 使用光规范化的SplitLUC试验,为研究植物PPI提供了可靠的定量方法.
  • 这种方法适用于弱相互作用,桥梁蛋白效应和域映射.