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Updated: Jan 9, 2026

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Circ_0001741通过miR-194-5p/E2F3轴调节ESCC中的扩散和入侵
Yuanyuan Wang1,2, Qianqian Sun3, Zhuyao Li4
1Clinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450001, China. wangyy224@zzu.edu.cn.
World journal of surgical oncology
|December 10, 2025
概括
这项研究表明,circ_0001741促进食道状细胞癌 (ESCC) 的进展. 准这种循环RNA可能为ESCC治疗提供了一个新的治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 胃肠病学 胃肠病学
背景情况:
- 食道状细胞癌 (ESCC) 是一种侵袭性癌症.
- 循环RNAs (circRNAs) 越来越多地被认为是癌症中的角色.
- 在ESCC中circ_0001741的特定功能仍然在很大程度上是未知的.
研究的目的:
- 调查circ_0001741在ESCC进展中的作用.
- 阐明ESCC中circ_0001741的潜在分子机制.
主要方法:
- 通过RNase R和actinomycin D测定证实了circ_0001741的圆形结构.
- 通过组织微阵列评估ESCC组织中的circ_0001741表达.
- 通过CCK-8和Transwell试验评估circ_0001741对细胞增殖和侵入的功能影响.
- 通过使用双露西法酶记者,RNA下拉和救援实验,研究了circ_0001741,miR-194-5p和E2F3之间的相互作用.
主要成果:
- 在ESCC组织中,Circ_0001741显著上调 (增加7.5倍).
- 沉默circ_0001741抑制了ESCC细胞的增殖和入侵.
- Circ_0001741作为miR-194-5p的分子海绵,对其目标瘤基因E2F3.3进行上调.
- 救援实验证实了ESCC进展中的功能circ_0001741/miR-194-5p/E2F3轴.
结论:
- Circ_0001741通过调节miR-194-5p/E2F3轴来促进ESCC的进展.
- Circ_0001741代表了ESCC治疗的潜在治疗目标.
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