在急性深静脉血栓症中通过核磁共振光谱学访问改变的代谢概况
Letícia Queiroz da Silva1, Thyerre Santana da Costa2, Lucas Gelain Martins2
1Hemocentro, Haemostasis and Inflammation Laboratory, Universidade Estadual de Campinas-UNICAMP, Campinas 13083-970, SP, Brazil.
International journal of molecular sciences
|December 11, 2025
概括
深静脉血栓 (DVT) 患者表现出持续的代谢变化,包括降低葡萄糖和增加乳酸,表明持续的组织缺氧和内皮功能障碍,即使在六个月后.
科学领域:
- 生物化学 生物化学
- 血管生物学 血管生物学
- 代谢学 代谢学 代谢学
背景情况:
- 深静脉血栓塞 (DVT) 需要新的生物标志物和风险因素识别.
- 代谢物分析为了解DVT病理生理学提供了一个有希望的途径.
- 目前对DVT中代谢变化的理解是有限的.
研究的目的:
- 用1H-NMR光谱学对急性DVT患者的代谢变化进行表征.
- 评估这些代谢变化的持续性在六个月的随访期间.
- 为了将代谢资料与炎症和内皮细胞标志物相关联.
主要方法:
- 从30名急性DVT患者和健康对照组的血清样本使用1H-NMR光谱分析.
- 血样本使用Luminex套件对10个标记物进行了评估.
- 在急性DVT,随访DVT (6个月) 和健康对照组之间比较了代谢概况.
主要成果:
- 急性DVT患者表现出谷氨酸,葡萄糖和分支链氨基酸 (BCAA) 的降低,乳酸的增加.
- 炎症标志物 (IL-6,IL-8) 急剧升高,但在随访时下降.
- 附着分子 (sVCAM-1,sICAM-1) 在六个月后增加,表明持续的内皮功能障碍.
- 在随访时,新陈代谢资料仅显示部分正常化.
结论:
- 代谢失调,包括葡萄糖代谢受损和乳酸增加,在急性DVT后持续很长时间.
- 粘附分子的持续升高表明正在进行的内皮功能障碍.
- 慢性代谢压力可能是由于内皮损伤,低度炎症或DVT后改变的线粒体功能造成的.
相关概念视频
01:20Venous Thrombosis II: Clinical Manifestations and Diagnostic Studies
270
The key difference between Superficial Vein Thrombosis (SVT) and Deep Vein Thrombosis (DVT) lies in their location and severity.Clinical ManifestationsSVT typically presents with localized pain, tenderness, and redness along the course of a superficial vein, often accompanied by a palpable, cord-like structure under the skin. This condition is usually less dangerous than DVT but can be uncomfortable and may lead to complications such as cellulitis or, rarely, a clot extension into the deep...
270
01:25Applications Of NMR In Biology
4.4K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.4K
01:07Nuclear Magnetic Resonance (NMR): Overview
6.6K
Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
6.6K