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在HEK-293细胞中HLA-A*03:01表达的特定不稳定性

María Area-Navarro¹, Alba Pastor-Moreno¹, Erika Scholz¹

¹Department of Cell Biology, Physiology and Immunology, Institute of Biotechnology and Biomedicine, Autonomous University of Barcelona, 08193 Bellaterra, Spain.

International journal of molecular sciences

|December 11, 2025

概括

HEK-293F细胞是一种HEK-293的变体,失去了HLA-A*03:01的表达,使他们不适合研究HLA-A*03:01连接体. 建议对HEK-293衍生细胞进行HLA-A*03:01的定期监测.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
质谱测量质量谱测量

背景情况:

HEK-293细胞是人类蛋白质表达的标准细胞系.
像HEK-293F这样的悬浮生长变体用于大规模细胞培养,对于免疫皮群净化至关重要.
HEK-293细胞系表达人类白细胞抗原 (HLA) I类全型,包括HLA-A*02:01,HLA-A*03:01,HLA-B*07:02和HLA-C*07:02.

研究的目的:

分析和比较 HEK-293 和 HEK-293F 细胞的 HLA I 类免疫.
为了研究这些细胞系中HLA I类异型的细胞表面表达.
确定HEK-293F细胞适用于涉及HLA-A*03:01.01的研究的适用性.

主要方法:

来自HEK-293和HEK-293F细胞的HLA-I免疫的净化和质谱分析.
流细胞计使用等位基特异体抗体量化细胞表面HLA-I全型表达.
产生一种表达β5i蛋白酶子单元和SARS-CoV-2尖端蛋白的HEK-293转菌剂.

主要成果:

HEK-293细胞的HLA-I免疫组含有来自所有表达的HLA-I全型的.
在HEK-293F细胞中,免疫组中缺乏来自HLA-A*03:01的.
流细胞计证实,HEK-293F细胞上没有表面HLA-A*03:01的表达.
一种产生的变质剂也表现出HLA-A*03:01表达的选择性丧失,这表明HEK-293系的趋势.

结论:

HEK-293F细胞不适合识别HLA-A*03:01配体或仅限于该等位基因的T细胞反应研究.
在HEK-293F细胞中选择性丧失HLA-A*03:01表达,需要在HEK-293-衍生细胞系中定期监测这种全型.
这些发现强调了验证免疫学研究的细胞系模型中HLA全型表达的重要性.

关键词:

据报道,HEK-293是该公司的首席执行官.哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈抗原呈现的呈现方式.免疫皮组 (immunopeptidome) 是一个免疫皮组.酸是一种酸.

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