毛囊内IL-10,孕和牛卵细胞发育能力之间的相关性
Aleksandra Teresa Pytel1,2, Dawid Tobolski1, Piotr Skup2
1Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-776 Warsaw, Poland.
International journal of molecular sciences
|December 11, 2025
概括
毛囊液中的介质素-10 (IL-10) 预测了在体外胚胎生产 (IVEP) 中牛卵细胞发育的成功. 较高的IL-10水平显著增加了囊胞发育的机会,提高了IVEP的效率.
科学领域:
- 生殖生物学 生殖生物学
- 生物技术是生物技术.
- 动物科学动物科学
背景情况:
- 卵细胞发育能力对于成功的牛体内胚胎生产 (IVEP) 是至关重要的.
- 确定可靠的卵细胞质量生物标志物对于提高IVEP效率至关重要.
- 目前评估卵细胞潜力的方法存在局限性.
研究的目的:
- 研究毛囊内白蛋白-10 (IL-10) 和孕激素 (P4) 度,毛囊特征和牛卵细胞发育能力之间的关系.
- 评估IL-10作为IVEP中卵细胞发育成功的预测生物标志物.
- 评估表面等离子体共振成像生物传感器对IL-10量化的实用性.
主要方法:
- 从FSH刺激的公牛身上收集了毛囊液和积卵细胞复合体 (n=314).
- 通过表面等离子体共振成像生物传感器量化了内IL-10.
- 孕激素 (P4) 通过酶相关光测定进行测量.
- 卵子细胞在体外培养以评估裂变和胚胎细胞形成率.
主要成果:
- 毛囊内IL-10度与胚胎细胞发育率正相关 (ρ = 0.29,p = 0.016).
- 来自IL-10> 142.16 pg/mL的卵泡中的卵细胞具有16.33倍的胚胎细胞发育的机会 (p = 0.006).
- 一个IL-10和卵细胞形态的组合模型在预测胚胎细胞成功时显示出高准确度 (AUC = 0.724).
- 糟糕的卵细胞形态和大的卵泡体积与发育失败有关.
- 卵泡内P4与卵泡大小相关,而不是直接与胚胎发育相关.
结论:
- 卵泡内IL-10是预测牛卵细胞能力的强有力的生物标志物.
- 使用生物传感器技术对IL-10的量化可以提高IVEP程序的效率.
- 这项研究提供了一种新的方法来改善IVEP的卵细胞选择.
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