孕产妇补充黑激素调节胎盘DNA甲基化和基因表达在营养受限牛
Shiveeli Rajput1, Brittni Littlejohn2, Zully E Contreras-Correa3
1Department of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS 39762, USA.
International journal of molecular sciences
|December 11, 2025
概括
孕产妇的营养限制和黑激素补充对牛胎盘DNA甲基化和基因表达产生影响,其影响因性别而异. 黑素可能会以性别特定的方式影响胎儿发育.
科学领域:
- 牲畜中的生殖生物学和表观遗传学.
- 孕产妇营养和胎儿编程.
- 妊娠和发育的内分泌调节.
背景情况:
- 母亲的营养限制会改变胎儿的发育和长期健康.
- 黑色素是一种激素,在生殖和发育中具有潜在的作用.
- 表观遗传修饰,如DNA甲基化,是发育过程中基因调节的关键.
研究的目的:
- 为了研究母体营养限制和黑激素对DNA甲基化和基因表达的影响,在牛的胎盘细胞.
- 要确定这些效应是否在概念上是性别特异性的.
- 为了确定受这些干预影响的特定基因和途径.
主要方法:
- 使用了2x2的因子设计,适当的养 (ADQ) 或营养限制 (RES) 和黑素补充剂 (MEL) 或控制 (CON).
- 在怀孕的240日,从雄性和雌性受孕者身上收集了牛胎盘.
- 使用甲基MiniSeq-GWBS分析了DNA甲基化,使用RNA-Seq.分析了基因表达.
主要成果:
- 营养素限制 (RES-CON与ADQ-CON) 导致胎盘骨干细胞的DNA甲基化模式 (DMR) 发生显著变化.
- 黑素补充剂在男性和女性胚胎中改变了甲基化模式,影响了像轴突引导和BDNF信号传递这样的途径.
- 基因表达分析揭示了性别特异性差异,其中黑激素对特定基因 (例如,UBOX5,PIGX,ATP11C) 进行上调,以应对营养限制.
结论:
- 孕产妇的营养限制和黑激素显著影响牛胎盘骨中的DNA甲基化和基因表达.
- 这些影响是性别特异的,表明男性和女性胎儿的不同编程.
- 黑素可能在调节胎儿的生长和发育方面发挥作用,特别是在营养限制条件下,以性别依赖的方式.
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