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多目标植物复合物通过双向P21/P53/SIRT1减轻细胞衰老规则:双模型验证

Jiaqin Wu¹, Nanjia Dongzhu², Chengzhou Zhao²

¹College of Pharmacy, Qinghai University, Xining 810016, China.

International journal of molecular sciences

|December 11, 2025

概括

一种植物复合物 (SBT) 抗击细胞衰老,这是一个关键的衰老因素. SBT增强了细胞活力和抗氧化防御,为与年龄相关的疾病提供了潜在的自然干预措施.

科学领域:

老年学和细胞生物学.
自然产品化学自然产品化学
老龄化的分子机制.

背景情况:

细胞衰老是衰老和与年龄相关的疾病的重要贡献者.
识别具有抗衰老特性的天然化合物对于开发干预措施至关重要.
现有的研究突出了了解衰老所涉及的分子途径的必要性.

研究的目的:

为了研究多组分植物复合物 (SBT) 的抗衰老作用.
阐明SBT抗衰老活性背后的分子机制.
在体外和体外衰老模型中评估SBT.

主要方法:

在H9c2心肌细胞中利用D-银糖诱导的衰老和在斑马鱼中利用H2O2诱导的加速衰老.
评估了细胞活力和与衰老相关的β-galactosidase (SA-β-gal) 活性.
分析了P21/P53/SIRT1信号轴,与亡相关的基因 (Bax,Bcl-2) 和抗氧化酶活动 (SOD,CAT,GSH,MDA).

主要成果:

在老化的细胞中,SBT显著改善了细胞活力,并减少了SA-β-gal活性.
SBT证明了P21/P53/SIRT1轴的双向调节,下调P53/P21和上调SIRT1.
在斑马鱼中,SBT通过增加SOD,CAT和GSH,同时降低MDA和调制的亡标志物 (Bax/Bcl-2) 来增强斑马鱼的抗氧化能力.

结论:

SBT表现出强大的抗衰老作用.
这些机制包括调节P21/P53/SIRT1通路,增强抗氧化防御和抑制亡.
SBT为基于天然产品的抗衰老疗法提供了机制基础.

关键词:

在 P21/P53/SIRT1 轴上灭症 (apoptosis) 是一种死亡的过程.双向监管是一种双向的监管.这是一个植物复杂的植物园.这是心肌细胞 (cardiomyocytes).细胞衰老细胞衰老多个目标的多重目标.疗法药物疗法药物疗法药物斑马鱼模型模型

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