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综合DNA甲基化和转录组分析揭示了克隆水牛哺乳性能差异的表观遗传机制

Jia-Hao Hu^1,2, Hai-Ying Zheng^1,2, Chun-Yan Yang^1,2

¹Guangxi Key Laboratory of Buffalo Genetics, Breeding and Reproduction, Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China.

International journal of molecular sciences

|December 11, 2025

概括

表观遗传调节会影响水牛的牛奶产量. 乳腺组织中的DNA甲基化模式在高和低产量的克隆水牛之间存在差异,影响基因表达和代谢途径.

科学领域:

* 乳制品科学乳制品科学
* 表观遗传学是一种表观遗传学.
* 基因组学是一个学科.

背景情况:

* 牛牛奶生产对乳制品行业至关重要.
* 牛奶产量受转录和表观遗传因素的影响.
*表观遗传机制驱动基因相同的水牛的牛奶产量变化尚未得到充分理解.

研究的目的:

* 调查DNA甲基化和基因表达在水牛牛奶产量变化中的作用.
* 利用克隆的水牛模型来研究表观遗传调节.
* 整合全基因组二硫酸盐测序 (WGBS) 和RNA测序 (RNA-seq) 数据.

主要方法:

* 全基因组双硫酸盐测序 (WGBS) 用于分析DNA甲基化.
*RNA测序 (RNA-seq) 用于评估基因表达.
*高产和低产克隆水牛之间的比较分析.

主要成果:

*低产量水牛在哺乳动物组织中显示全球DNA甲基化减少.
*不同甲基化的基因与PI3K-Akt,HIF-1和免疫通路有关.
*高产水牛上调脂质代谢和细胞增殖基因;低产水牛显示免疫应激和氨基酸代谢丰富.
*综合性分析确定了126个低调控基因和关键调控因子,如KLF6和ESR1.

结论:

*为克隆水牛的乳产量变化建立了初步的多学科监管格局.
* 证据表明DNA甲基化和基因转录之间的相互作用在调节牛奶产量方面起作用.
*确定了分子通路和调节剂,为未来研究改善水牛牛奶生产提供了目标.

关键词:

通过DNA甲基化.克隆的水牛克隆的水牛表观遗传调节表观遗传调节牛奶产量变化牛奶产量变化转录组 (transcriptome) 是一个转录组.

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