在现场进行半定量微流体检测,以检测尿液中的11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH)
YeJi Jung1, Isaac Choi2,3, Hyunjun Bae3
1Department of Forensic Sciences, Sungkyunkwan University, Suwon 16419, Republic of Korea.
Sensors (Basel, Switzerland)
|December 11, 2025
概括
一个新的便携式设备可以快速,灵敏和客观地检测尿液中的大麻代谢物THC-COOH. 这种免疫测试可以在没有预处理的情况下准确识别THC-COOH,从而提高了法医药物测试能力.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 生物医学工程 生物医学工程
背景情况:
- 准确检测11-nor-9-carboxy-delta-9-tetrahydrocannabinol (THC-COOH),一个主要的大麻代谢物,对于药物测试至关重要.
- 传统的免疫试验往往在THC-COOH检测的敏感性和客观性方面扎.
研究的目的:
- 开发一个便携式的侧流免疫测试装置,用于尿液中半定量THC-COOH检测.
- 通过使用测试对参考光率来减轻矩阵效应来确保客观和可靠的结果.
主要方法:
- 开发一种微流体弹和光读取器,用于快速检测THC-COOH.
- 使用尖尿样进行验证,并对100个真实尿样进行LC-MS/MS的比较.
- 使用截止指数 (COI) 进行客观的结果解释.
主要成果:
- 该设备在20 ng/mL的截止值实现了100%的灵敏度和特异性.
- 可重复性和可重复性显示变化系数 (CVs) 低于15%.
- 在COI和LC-MS/MS结果之间观察到强烈的相关性 (R2 = 0.9471),表明识别了自由和结合THC-COOH.
结论:
- 微流体免疫检测提供了一种快速,灵敏和客观的方法,用于现场检测THC-COOH.
- 该设备在没有预处理的情况下检测结合代谢物的能力提高了其在法医应用中的可靠性.
- 通过Wi-Fi连接自动化数据管理进一步提高了其法医适用性.
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