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低成本的旋盘共聚焦显微镜,使用25兆像素摄像头

Guy M Hagen¹, Brian Lewis¹, Summer Levis¹

¹UCCS BioFrontiers Center, University of Colorado Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USA.

Sensors (Basel, Switzerland)

|December 11, 2025

概括

我们使用高分辨率的CMOS摄像头开发了一种低成本的旋盘共聚焦显微镜. 这种负担得起的系统为生物样本提供了先进的3D成像功能,使复杂的显微镜可访问.

科学领域:

生物物理学的生物物理.
显微镜技术显微镜技术
光学工程是指光学工程.

背景情况:

旋盘共聚焦显微镜提供快速的光学切割和低光毒性.
高昂的硬件成本限制了先进的显微镜技术的可用性.
现有的系统通常依赖于昂贵的科学CMOS (sCMOS) 摄像头.

研究的目的:

开发一个具有成本效益的旋盘共聚焦显微镜解决方案.
在这个设置中评估机器视觉CMOS摄像机的性能.
为了展示一个开放的访问和可扩展的替代先进的成像.

主要方法:

使用了一台25兆像素的机器视觉CMOS摄像头,带有反向照明传感器.
建造了一个定制的旋转盘,有18微米的针孔.
集成硬件与Micromanager软件进行同步控制.
在小鼠大脑切片,果虫标本和老鼠丸上测试成像性能.

主要成果:

实现了各种生物组织的高分辨率3D成像.
测量空间分辨率为215.8nm (X,Y) 和521.9nm (Z) 的60×/1.42 NA镜头.
使用自定义磁盘,在较低的放大度下证明了稳定,近距离的成像.

结论:

价格实惠的CMOS摄像头可以有效地取代旋盘显微镜中的sCMOS传感器.
开发的系统为先进的生物成像提供了一个可扩展和可访问的解决方案.
这一创新降低了高性能共聚焦显微镜的进入壁垒.

关键词:

这是一个CMOS摄像头.同焦点显微镜的共焦显微镜.光是一种光.一个旋转的盘子

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