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导向AtMP2的进化:释放来自Anabas testudineus的增强抗菌潜力

Li Ting Lee¹, Arnold Ang¹, Ahmed Najm^2,3

¹Faculty of Health and Life Sciences, Inti International University, Nilai 71800, Negeri Sembilan, Malaysia.

Molecules (Basel, Switzerland)

|December 11, 2025

概括

来自爬 (Anabas testudineus) 的修饰抗菌显示出对具有较低细胞毒性的阳性细菌的抗菌活性增强. 这些新型为各种治疗应用提供了传统抗生素的潜在替代品.

科学领域:

生物化学和分子生物学
药理学药理学是指药理学的学科.
海洋生物学海洋生物学

背景情况:

抗微生物 (AMP),如从爬山 (Anabas testudineus) 中获得的AtMP1和AtMP2,具有抗菌和抗癌特性.
现有的AMP具有潜力,但需要优化以提高有效性和安全性.
皮粘液的Anabas testudineus是一种生物活性的来源.

研究的目的:

通过系统定向进化增强AtMP2的抗菌活性.
评估修改的AtMP2变体的抗菌疗效和细胞毒性.
为了研究改性与细菌标蛋白的结合相互作用.

主要方法:

使用系统定向进化的AtMP2变体的生成.
使用APD3,CAMP和AMPFUN的变体的表征.
通过最小抑制度 (MIC) 和Kirby-Bauer盘扩散进行抗菌活性评估.
使用SRB测定对HS-27和Vero细胞系进行细胞毒性评估.
使用ZDOCK和HPEPDOCK服务器进行分子对接模拟.

主要成果:

与原生AtMP2相比,两种合成的变体AtMP2-1和AtMP2-2显示出对格拉姆阳性细菌的优越抗菌活性.
在20μg/mL时,AtMP2-1和AtMP2-2对人类细胞系没有显著的细胞毒性.
对接分析显示,AtMP2-1和AtMP2-2对Seca,RpoB和GyrA等重要细菌蛋白质具有强烈的结合亲和力.

结论:

来自Anabas testudineus的修改AtMP2 (AtMP2-1,AtMP2-2) 具有增强的抗菌特性,并且对人类使用安全.
这些代表了开发抗细菌感染的新型治疗剂的有希望的候选人.
对它们的特定作用机制进行进一步的研究是有必要的,以便在伤口愈合和癌症治疗中的应用.

关键词:

阿纳巴斯是我们的学生.抗微生物类的抗微生物.药物耐药性耐药性药物耐药性鱼类蛋白质鱼类蛋白质鱼类蛋白质人类健康人类健康人类健康

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