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尼古丁胺胺单核酸通过在液体储存期间维持线粒体功能来提高野猪精子质量

Yongjin Liu¹, Hongyan Zhang¹, Qingzhe Meng¹

¹College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.

Animals : an open access journal from MDPI

|December 11, 2025

概括

尼古丁胺胺单核酸 (NMN) 补充剂在储存期间改善了野猪精子质量. NMN增强了精子的运动性,完整性和受精潜力,提供了一个有希望的保存策略.

科学领域:

生殖生物学生殖生物学
生物化学生物化学
动物科学动物科学

背景情况:

尼古丁胺胺单核酸 (NMN) 对于NAD+合成和细胞能量至关重要.
在液体储存期间,野猪精子质量下降,影响生育能力.
了解NMN在精子代谢中的作用对于保存至关重要.

研究的目的:

调查NMN补充剂在液体储存期间对野猪精子质量的影响.
确定最佳的NMN度,以保持野猪精子的生命力和功能.
探索NMN对猪精中的细胞内NAD+和ATP水平的影响.

主要方法:

猪精液被稀释在Modena扩展剂中,NMN度不同 (0-80μM).
精子质量参数 (流动性,体完整性,线粒体活性) 在17°C保存7天后进行评估.
测量了细胞内NAD+和ATP水平.
西方斑点分析确定了NMNAT3的表达.
进行了蛋白质氨酸酸化,电容率和精子-卵管相互作用测试.

主要成果:

补充20μM的NMN显著改善了精子运动性,体完整性和线粒体活性.
在接受NMN治疗的精子中,细胞内NAD+和ATP水平明显升高 (p <0.05).
NMN治疗增强了蛋白质氨酸酸化,电容率和精子-卵管相互作用.

结论:

20μM NMN 补充剂在液体储存期间有效地保持了野猪精子的代谢活性和功能能力.
通过增加细胞内NAD+和ATP水平,NMN可以提高精子质量.
NMN为改善野猪精液保存和生育结果提供了一个有希望的策略.

关键词:

野猪野猪野猪野猪野猪电容容量是指电容的容量.线粒体功能线粒体功能尼古丁胺胺 mononucleotide 的使用情况精子质量精子质量精子质量

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