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MSTN基因淘汰对中国美利诺羊 (新疆类型) 的生长性能和肌肉转录组的影响

Li Zhang^1,2, Pengfei Li^1,2, Xu Wang^1,2

¹College of Animal Science, Xinjiang Agricultural University, Urumqi 830091, China.

Animals : an open access journal from MDPI

|December 11, 2025

概括

肌静素 (MSTN) 基因淘汰显著提高了中国美利诺羊的生长性能. 基因编辑改善了体重和体型,而不会影响健康,为肉羊养殖提供了洞察力.

科学领域:

动物遗传学动物遗传学
分子生物学分子生物学
畜牧科学畜牧科学畜牧科学

背景情况:

肌平素 (MSTN) 基因是肌肉发育的关键调节者.
在各种动物模型中,MSTN敲击已经证明了增长的增强.
中国美利诺羊 (新疆类型) 用于研究MSTN在肌肉生长中的作用.

研究的目的:

为了评估MSTN淘汰中华美利诺羊的生长性能,血液学和生物化学参数.
通过转录组分析识别受MSTN淘汰影响的关键基因和途径.
为肉羊的基因编辑育种策略提供基础.

主要方法:

在MSTN淘汰赛和对照羊之间比较生长特征 (体重,长度,身高).
血液学和生物化学参数的分析,以评估动物健康.
转录组分析以识别差异表达基因 (DEG) 并构建蛋白质-蛋白质相互作用 (PPI) 网络.
关键鉴定基因的RT-qPCR验证.

主要成果:

MSTN淘汰赛羊的体重,身长和身高显著改善.
在血液学或生物化学参数上没有观察到任何不良影响.
转录组分析显示,包括氨基酸代谢,肌肉收缩和免疫反应在内的途径中有121个DEG.
一个PPI网络确定了10个潜在由MSTN调节的核心基因.

结论:

"MSTN淘汰"是一种可行的策略,可以提高中国美利诺羊的生长表现.
通过MSTN淘汰进行基因编辑不会影响动物的健康或代谢状态.
这项研究为开发基因编辑肉类绵羊品种提供了宝贵的理论和实践见解.

关键词:

中国美利诺羊 (新疆类型)肌静止素 (MSTN) 是一种转录组 (transcriptome) 是一个转录组.

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