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替代分离事件对蒙古马的精子生成的影响
Dailing Song1,2,3, Guoqing Wang1,2,3, Terigele Baterin1,2,3
1Key Laboratory of Equus Germplasm Innovation (Coconstruction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Hohhot 010018, China.
Animals : an open access journal from MDPI
|December 11, 2025
概括
MEI1基因的替代拼接,特别是相互排斥的外因子 (MXE) 和跳过的外因子 (SE) 事件,影响蒙古马精子生成. 无论是MXE还是SE事件,都通过调节基因和代谢物表达来促进精子生成.
科学领域:
- 生殖生物学 生殖生物学
- 分子遗传学 分子遗传学
- 马类科学 马类科学
背景情况:
- 蒙古马的精子生成涉及线粒体和介质体的分裂.
- MEI1基因对于平移过程中的正常染色体结合至关重要.
- 替代性拼接MEI1可能会影响蒙古马精子生成.
研究的目的:
- 研究MEI1替代拼接事件对蒙古马精子生成的调节效应.
- 确定MEI1的相互排斥的外因子 (MXE) 和跳过的外因子 (SE) 事件如何影响精子生成.
主要方法:
- 在塞尔托利细胞中构建和利用MEI1 MXE和SE事件的lentiviral载体.
- 采用光量化,转录学和代谢学来分析基因和代谢物表达.
- 选和注释差异表达的基因和代谢物.
主要成果:
- 与MXE事件相比,在SE事件中观察到更高的MEI1表达 (p < 0.001).
- 鉴定了193个差异表达的基因和11,360个差异表达的代谢物,这些代谢物丰富于精子生成途径.
- 在SE和MXE事件之间观察到不同的基因 (例如IL31RA,IL11) 和代谢物 (例如叶酸,酸) 表达模式.
结论:
- MEI1 MXE和SE剪接事件通过调节相关基因和代谢物来影响精子生成.
- 这两种拼接事件都促进了精子生成信号通路.
- 这些发现为了解马精子生成中的MEI1替代拼接调节提供了基础.
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