Enterococcus faecium WEFA23-derived Surface Layer Protein OTC 通过加强肠道屏障功能和调节免疫反应来防止Listeria monocytogenes的入侵
1State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, China.
Foods (Basel, Switzerland)
|December 11, 2025
概括
来自Enterococcus faecium的表面层蛋白甲甲基转移酶 (OTC) 抑制了Listeria monocytogenes的入侵. OTC增强了肠道屏障功能,调节了宿主免疫反应,为抗感染策略提供了潜力.
科学领域:
- 微生物学和免疫学
- 食品科学与技术 食品科学与技术
- 胃肠病学 胃肠病学
背景情况:
- 李斯特菌 (Listeria monocytogenes) 是一种重要的食源性病原体,通过入侵肠道细胞引起严重感染.
- 益生菌及其表面蛋白质在加强肠道屏障和防止病原体入侵方面表现有前途.
研究的目的:
- 为了评估Enterococcus faecium WEFA23的表面层蛋白甲甲基转移酶 (OTC) 对Listeria monocytogenes的对抗作用.
- 研究OTC在抗粘附,抗入侵,炎症反应,肠道屏障完整性和转录基因变化的作用.
主要方法:
- 在体外研究中使用人类肠道上皮Caco-2细胞.
- 野生类型的Enterococcus faecium WEFA23与OTC基因淘汰菌株 (E. faecium WEFA23 otc-/-) 的比较.
- 对抗粘附,抗入侵,细胞因子反应,屏障完整性和RNA测序 (RNA-seq) 的评估,用于转录组分析.
主要成果:
- 在OTC的存在下,Enterococcus faecium WEFA23表现出明显更强的抗粘附和抗入侵能力对L. monocytogenes (p <0.05).
- 通过增加密集结蛋白表达,OTC可能会增强肠道屏障的完整性,并调节炎性细胞因子.
- RNA-seq确定了参与GABA-活性突触,和通类受体信号通路的差异表达基因 (DEG),这表明了OTC的作用机制.
结论:
- 来自Enterococcus faecium WEFA23的OTC在抑制Listeria monocytogenes入侵方面发挥着至关重要的作用.
- 通过调节宿主信号通路和病原体毒性,OTC有助于抗微生物防御和肠道粘膜保护.
- 这些发现支持开发新的基于益生菌的抗感染干预策略.
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