走向实用和灵敏的光分析:因子设计优化的o-Phthalaldehyde衍生物化,用于同步的光谱度测定莫达菲尼尔在血和剂量形式
Yara Y Ali1, Nermine V Fares1, Miriam F Ayad1
1Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Abassia, Cairo, Egypt.
Luminescence : the journal of biological and chemical luminescence
|December 11, 2025
概括
一种新的光谱计量方法准确地分析莫达菲尼尔 (MDF),一种用于治疗ALS疲劳的药物. 这种敏感的技术在药物和生物样本的常规分析中很实用.
科学领域:
- 分析化学 分析化学
- 药品分析 药品分析
背景情况:
- 莫达菲尼尔 (MDF) 处方用于管理与肌缩侧面硬化症相关的疲劳.
- 准确量化MDF对于治疗监测和质量控制至关重要.
研究的目的:
- 开发和验证一种简单,灵敏和实用的光谱测量方法,用于分析莫达菲尼尔 (MDF).
- 评估该方法在制药配方和生物矩阵中的适用性.
主要方法:
- 在MDF和o-phthalaldehyde之间发生的基催化凝结反应形成了一个光的isoindoline衍生物.
- 用优化参数 (Δλ = 110 nm) 的同步光谱法测量用于在445 nm的检测.
- 方法验证遵循国际协调会议的指导方针,包括线性,回收,LOD和LOQ的确定.
主要成果:
- 该方法在广泛的度范围 (107000 ng/mL) 上表现出极好的线性.
- 对于MDF,无论是药片配方 (100.43% ± 0.16%) 还是的人体血 (96.07% ± 1.55%) 的平均恢复率都很高.
- 检测极限 (LOD) 为3.21 ng/mL,量化极限 (LOQ) 为9.74 ng/mL.
结论:
- 开发的光谱计量方法具有高度灵敏性,准确性,并且适合用于常规的莫达菲尼尔定量分析.
- 它的用户友好性和在等离子体等复杂矩阵中证明的有效性支持其在临床和制药环境中的应用.
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