相关实验视频

Updated: Jan 9, 2026

Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication
05:55

Author Spotlight: Unveiling the Role of SNF2L in Replication Fork Stability and Genome Duplication

Published on: August 23, 2024

976

性氧化 (BrdU) 彗星测试用于检测复制相关的DNA损伤

Diego Luis Ribeiro1, James Eduardo Lago Londero1, Davi Jardim Martins2

  • 1Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.

Current protocols
|December 11, 2025
PubMed
概括

这项研究详细介绍了一种优化的性BrdU彗星试验,用于检测新合成DNA链中的DNA损伤. 该方法专门测量了非同步的人类细胞中与复制相关的DNA断裂,有助于DNA修复研究.

关键词:
性 BrdU 彗星测定试验叉的进展动力学 叉的进展动力学基因组的不稳定性复制压力是复制的压力.复制性DNA损伤导致的DNA损伤.

更多相关视频

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

4.5K
A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage07:57

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage

Published on: May 10, 2022

5.8K

相关概念视频

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.9K