一种保存的菌根核模块素劫持了宿主指南针复合体,以重编程促炎转录并促进细胞内生存
Liu Chen1,2, Baojie Duan1, Pingping Chen1
1National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
结核菌菌使用一种核模块林,MgdE,以增强宿主细胞内的生存能力. MgdE针对COMPASS复合体,抑制炎症基因转录并促进感染.
科学领域:
- 细菌学 细菌学是一门学科.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 核模块是转移到宿主细胞核中的细菌蛋白质.
- 它们在菌根菌病原和分子机制中的作用在很大程度上是未知的.
- 了解这些相互作用对于开发新的治疗策略至关重要.
研究的目的:
- 为了识别和描述菌根菌中的新型核模块素.
- 阐明这些蛋白质促进细胞内生存的分子机制.
- 研究细菌核模块和宿主核机械之间的相互作用.
主要方法:
- 保存的假设蛋白质Rv1075c (MgdE) 作为核模块素的鉴定.
- 使用核定位信号 (NLS) 分析核转位.
- 同免疫沉测试以确定宿主蛋白相互作用.
- 定量PCR用于评估促炎性细胞因子的基因表达.
主要成果:
- Rv1075c,指定为MgdE,增强了菌根细菌的细胞内生存.
- MgdE通过已识别的NLS转移到宿主核中.
- MgdE与ASH2L和WDR5相互作用,它们是宿主COMPASS复合体的组成部分.
- 这种相互作用抑制了H3K4甲基化,并减少了IL6和IL1B的转录.
结论:
- MgdE是一种新型核模块素,可促进菌根菌的存活.
- MgdE直接针对宿主COMPASS复合体,以逃避免疫反应.
- 这项研究揭示了细菌病原和宿主免疫调节的新机制.
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