Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

9.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.2K
DNA Microarrays02:34

DNA Microarrays

20.6K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Rational design of an acridine-derived click chemistry-based artificial metallo-nuclease.

RSC chemical biology·2026
Same author

A Click Chemistry Approach for Developing Organelle-Directed Photocytotoxic Ruthenium(II) Probes.

Journal of medicinal chemistry·2026
Same author

Accessing New Phenanthroline-Oxazine Scaffolds as Copper-Dependent DNA Damaging Probes.

Chembiochem : a European journal of chemical biology·2026
Same author

Crystallographic binding modes of octahedral transition metal complexes to duplex DNA.

Quarterly reviews of biophysics·2026
Same author

Expanding the DNA damaging potential of artificial metallo-nucleases with click chemistry.

Nature communications·2026
Same author

From loops to caps: discriminating peptide binding to distinct G-quadruplex tetrads using 5-furyl-2'-deoxyuridine fluorescent probes.

RSC chemical biology·2025

相关实验视频

Updated: Jan 9, 2026

Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC Crosslinking of Small Molecules to Isolate Chromatin
10:05

Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC Crosslinking of Small Molecules to Isolate Chromatin

Published on: January 20, 2016

8.6K

最近在探测小分子与DNA相互作用方面取得了进展.

Simon Poole1, Bríonna McGorman1, Christine J Cardin2

  • 1School of Chemical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.

Biophysical reviews
|December 11, 2025
PubMed
概括

了解与非正规核酸的药物-DNA相互作用是开发向疗法的关键. 最近生物物理技术的进步有助于发现用于基因调节和基因组稳定性的新临床药物.

关键词:
这就是B-DNA.它们是DNA DNA DNA DNA.药物-DNA相互作用霍利代交叉口的节假日节点.非正典的DNA是非正典的DNA核酸是核酸中的一种.三路交叉路口是三路交叉路口.三重螺旋的三重螺旋形成三倍体的寡核酸.

更多相关视频

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.5K
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

12.0K

相关实验视频

Last Updated: Jan 9, 2026

Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC Crosslinking of Small Molecules to Isolate Chromatin
10:05

Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC Crosslinking of Small Molecules to Isolate Chromatin

Published on: January 20, 2016

8.6K
Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.5K
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

12.0K

科学领域:

  • 药用化学 医学化学
  • 分子生物学分子生物学
  • 生物物理学的生物物理.

背景情况:

  • 核酸是关键的治疗点.
  • 开发向疗法需要了解药物-DNA相互作用.
  • 非正规的核酸结构 (例如G-四重复,i-动机) 在基因调节和基因组稳定性中起作用.

研究的目的:

  • 审查最近在分析更高阶药物-DNA相互作用方面的进展.
  • 突出这些相互作用在向治疗的合理设计中的作用.
  • 讨论与非正规核酸相互作用的连接体和金属复合物的治疗潜力.

主要方法:

  • 频谱学方法 频谱学方法
  • 晶体学 晶体学是指结晶学.
  • 生物分子检测试验
  • 对药物-DNA相互作用的分析.

主要成果:

  • 新分子优先与复杂的核酸结构相互作用.
  • 描述与非正典结构的相互作用已经确定了有前途的治疗剂.
  • 生物物理技术对于探测这些相互作用至关重要.

结论:

  • 分析药物-DNA相互作用的进展有助于设计有针对性的治疗方法.
  • 针对非正规核酸为新型临床药物提供了潜在的潜力.
  • 了解这些相互作用对于药物发现至关重要.