在酸面乳酸细菌菌株子集中对活力和细胞稳定性的冷干燥效应
Valentina Musi1, Elisa Aiello1, Mattia Pia Arena1
1Department of Life Sciences, University of Modena and Reggio Emilia, Reggio Emilia, 42122, Italy.
Current microbiology
|December 11, 2025
概括
冷干燥协议显著影响乳酸细菌 (LAB) 的生命力和功能. 量身定制的保存策略对于保持培养集中的这些重要微生物资源的长期稳定性至关重要.
科学领域:
- 食品生物技术 食品生物技术
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 微生物资源对于食品生物技术至关重要,影响产品质量,工艺效率和安全.
- 长期保存对于在培养集 (CC) 和微生物生物资源中心 (mBRC) 中保持微生物培养至关重要.
研究的目的:
- 研究不同冷干燥预冷方案对乳酸细菌 (LAB) 菌株的影响.
- 在各种保存条件下比较LAB的活力,发酵性能,代谢物生产和细胞完整性.
主要方法:
- 使用微生物计数评估生存能力.
- 通过HPLC分析分析发酵性能和代谢物生产.
- 使用扫描电子显微镜 (SEM) 成像评估细胞完整性.
主要成果:
- 在所有测试条件下,Lactiplantibacillus plantarum UMCC 2996 显示出高生存率 (>95%) 和稳定的特征.
- 果杆菌圣弗朗西斯人UMCC 2990和Leuconostoc citreumUMCC 3011表现出显著的生存能力丧失和代谢概况的改变,特别是在-80°C的预冷方案下.
- 在敏感的LAB菌株中,SEM揭示了结构损伤,包括细胞碎片和膜纹.
结论:
- 冷干燥协议需要针对不同的LAB菌株进行定制,以确保它们的长期功能.
- 定制的保存策略对于在培养收藏和mbrcs中保持酸面粉衍生LAB的完整性和性能至关重要.
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