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在海黄瓜的性腺体中的差异表达miRNAs Holothuria leucospilota

Jing Zhang¹, Xiao-Qin Fu², Run-Xin Lin¹

¹School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, China.

Comparative biochemistry and physiology. Part D, Genomics & proteomics

|December 11, 2025

概括

研究人员在雄性和雌性海 (Holothuria leucospilota) 中鉴定出97个差异表达的微RNA (DEM). 这些发现为性别识别提供了分子标记,并对海性别差异化提供了洞察力.

科学领域:

海洋生物学海洋生物学
分子遗传学分子遗传学
发育生物学发展生物学

背景情况:

微RNAs (miRNAs) 是细胞分化,发育和性别决定的关键调节者.
海黄瓜 (Holothuria leucospilota) 在经济上是有价值的,但由于性别识别的挑战,很难繁殖.
识别用于确定性别的分子标记物对于提高海黄水产养殖效率至关重要.

研究的目的:

为了比较雄性和雌性海之间的miRNA表达特征.
识别可以作为性别识别分子标记的差异表达miRNAs (DEMs).
探索miRNA及其基因在海黄的性别差异化中的潜在作用.

主要方法:

使用高通量测序来分析雄性和雌性H. leucospilota.性腺组织中的miRNA表达.
进行了生物信息分析,以确定已知的和新的miRNAs,以及显著的DEMs.
构建了一个miRNA-mRNA调节网络,并对目标基因进行了途径分析. 使用定量实时PCR (RT-qPCR) 进行验证.

主要成果:

总共有584个已知的和404个新的miRNAs被确定.
97个miRNA在雄性和雌性海之间显著差异地表达.
途径分析表明神经活性联体受体相互作用,信号传递和MAPK信号传递在性别差异化中的参与.

结论:

这项研究确定了参与H. leucospilota.性别差异化的关键miRNAs.
鉴定的DEM提供了潜在的分子标记物来区分性别,有助于水产养殖.
这些发现为未来研究miRNA介导的海性别调节奠定了基础.

关键词:

戈纳德·戈纳德 (Gonad Gonad) 是一个霍洛图里亚白细胞飞行者海黄瓜的海黄瓜是什么意思它们是mRNARNA.这是一个小RNARNA.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...