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Updated: Jul 12, 2026

Bromodeoxyuridine BrdU Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton
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在压载沉积物中优化可活性的细菌检测:解决PCR抑制剂和遗留DNA

Haoze He1, Zhaozhao Xue1, Zhenghua Xu2

  • 1Marine College, Shandong University, Weihai, China.

Ecotoxicology and environmental safety
|December 11, 2025
PubMed
概括

准确监测压载中的活力微生物需要解决遗留DNA和PCR抑制. 结合单 (PMA) 治疗和抑制剂去除对于可靠的微生物社区分析至关重要.

关键词:
细菌多样性的细菌多样性压载沉积物的沉积物.PCR 抑制剂 PCR 抑制剂普罗皮单化物 (Propidium monoazide) 是一种有生命力的细菌.

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Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation09:49

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DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...