脱氧尼瓦伦醇和铜的相互作用:小鼠肝细胞中的细胞和分子洞察力
Hui Jiang1, Junhua Yang2, Xichun Wang1
1College of Veterinary Medicine, Anhui Agricultural University, Hefei, 230036, China.
概括
脱氧尼瓦伦 (DON) 和铜 (Cu) 污染食品,对健康构成风险. 这项研究发现,虽然两者都会导致细胞损伤,但铜可以减轻 DON DON 的作用.
科学领域:
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 脱氧尼瓦伦 (DON) 和铜 (Cu) 是重要的食品污染物,对动物和人类的健康有潜在影响.
- 肝细胞是有毒物质的主要点,因为它们在新陈代谢和排毒中的作用.
研究的目的:
- 研究DON和Cu对小鼠肝细胞 (NCTC1469细胞) 的单独和联合毒理作用.
- 阐明所观察到的细胞反应背后的分子机制,包括炎症和自途径.
主要方法:
- 对暴露于DON和Cu的NCTC1469细胞进行细胞活力测定和超结构分析.
- 测量促炎性细胞因子 (IL-1β,TNF-α) 和抗氧化酶活性 (谷氨,SOD).
- 转录组测序和西部斑分析,以评估与自相关的基因表达和蛋白质水平 (LC3-II/LC3-I,p62).
主要成果:
- DON和Cu都诱导了细胞损伤,线粒体真空化,炎症,减少了细胞活力,并抑制了抗氧化酶.
- 与单独使用DON相比,与DON+Cu同时接触提高了细胞活力和降低了IL-1β水平.
- 转录组分析显示显著的基因表达变化,丰富了与自相关的途径;升高的LC3-II/LC3-I和降低的p62表明激活自.
结论:
- 在小鼠肝细胞中表现出毒性,诱导细胞损伤和炎症.
- 铜在低度下减轻了DON诱导的肝毒性,可能通过调节与Beclin-1/p62相关的自.
- 这些发现突出了DON和Cu毒性之间的复杂相互作用,以及自在细胞防御机制中的作用.
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