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过度表达肝细胞生长因子的转基因MSC用于治疗人类耐火性伤口

Min Nie¹, Danqing Huang¹, Dandan Wang¹

¹Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210002, China.

Science bulletin

|December 11, 2025

概括

在水凝中过度表达肝细胞生长因子 (HGF) 的工程介质细胞 (MSC) 有效地治疗自身免疫患者的耐火性伤口. 这种新疗法通过增强细胞功能和提供长效疗效来促进伤口愈合.

科学领域:

再生医学是一种再生医学.
细胞疗法细胞疗法
生物材料是一种生物材料.

背景情况:

耐火性伤口 (RWs) 是一个重要的临床挑战,治疗选择有限.
肝细胞生长因子 (HGF) 在自身免疫性疾病下调,这表明它在受损伤愈合中的作用.

研究的目的:

开发和评估一个充满过度表达HGF (MSC^HGF) 的基因工程介质层细胞 (MSC) 的可注射水凝系统,用于治疗自身免疫患者的RW.

主要方法:

介酶体 stromal 细胞被基因改造以使用电穿孔过度表达 HGF.
工程MSC被装入可注射水凝中进行持续的输送.
用MSC^HGF加载的水凝的治疗疗效在患有自身免疫疾病和RWs的患者中进行了评估.

主要成果:

通过c-Met/PI3K/AKT通路,MSC^HGF表现出稳定的HGF表达和分泌,增强的迁移和改善的抗亡.
从MSC^HGF分泌的HGF和其他因素促进了血管内皮细胞迁移和血管生成,减少了巨细胞炎症,并改善了纤维细胞细胞外基质重塑.
装有MSC^HGF的水凝显著改善了细胞功能,并在临床自身免疫患者中提供了长效的伤口愈合.

结论:

通过可注射水凝输送的基因工程MSC^HGF代表了在自身免疫疾病中耐火性伤口的有前途的治疗策略.
这种方法通过利用细胞工程和生物材料传递系统,为推进伤口治疗提供了临床意义.

关键词:

在HGF的基础上.水凝是一种水凝.介质细胞层细胞的介质细胞层细胞.耐火性伤口的伤口伤口愈合伤口愈合

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