生物相容的基于硫的共价探针用于活细胞和固定细胞中的内源性管氨酸光纳米镜
Marie Auvray1, Tanja Koenen2, Olexandr Dybkov3
1Chromatin Labeling and Imaging group, Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
Nature communications
|December 11, 2025
概括
研究人员开发了一种新的光探针,用于检测细胞骨中的关键蛋白质 - - 氨酸. 这种最小侵入性探针可以在不破坏蛋白质功能的情况下对细胞过程进行高分辨率成像.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 显微镜的使用方法
背景情况:
- 光探针对于可视化细胞动态至关重要,特别是超分辨率显微镜.
- 传统的标签方法,如光蛋白融合或联结光体,可能会干扰蛋白质的功能并引起副作用.
研究的目的:
- 开发一种新型的,最少侵入性的共振光探针,用于内源性管.
- 为了实现细胞骨动态的高分辨率成像,而不会影响管的功能.
主要方法:
- 使用cabazitaxel和-rhodamine的共价光探针 (6-SiR-o-C9-CTX) 的设计和合成.
- 加入一个生物相容的可切割连接器与一个硫中心.
- 在活细胞和固定细胞中使用STED纳米镜验证多个人类细胞系中的探针性能.
主要成果:
- 探测器证明了细胞透性,化行为,以及管素的高效共价标记.
- 标记后可以去除对分类向的部分,从而保持管的功能.
- 在STED纳米镜中成功应用,用于高分辨率的细胞骨成像.
结论:
- 开发的光探针为研究管动力学提供了一种最少的侵入性方法.
- 这种技术提高了观察高精度动态细胞过程的工具包.
- 该探头可提供高分辨率的功能性图像,对细胞骨进行成像.
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