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实时PCR测定用于对Mycobacterium tuberculosis Haarlem基因型进行可靠的检测和全球监测

Daria Tkachuk¹, Anna Vyazovaya¹, Irina Kostyukova²

¹St. Petersburg Pasteur Institute, St. Petersburg, Russia.

Scientific reports

|December 11, 2025

概括

一种新的实时PCR (RT-PCR) 试验可靠地检测出Mycobacterium tuberculosis的哈勒姆基因型,即使当 spoligotyping 失败时. 这种方法揭示了哈勒姆基因型在全球范围内比以前估计的更为普遍.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
流行病学流行病学

背景情况:

哈勒姆基因型是Mycobacterium结核病的研究不足的血统,具有明显的致病特征.
传统的spoligotyping方法难以准确识别哈勒姆基因型,特别是在具有广泛间距删除的隔离物中.

研究的目的:

开发和验证一项可靠的实时PCR (RT-PCR) 试验,用于特定检测哈勒姆基因型.
评估哈勒姆基因型在不同Mycobacterium结核病菌种群中的流行率.

主要方法:

设计了一种针对哈勒姆基因型特征的特定Rv0282 211 C>T突变的RT-PCR试验.
使用锁定核酸 (LNA) 探针来提高检测准确度.
优化和验证了使用已知全基因组测序 (WGS) 数据的68个分离物进行测定,随后对428个分离物的多样化集合进行应用.

主要成果:

RT-PCR试验与WGS数据具有很高的一致性,在428个分离物中准确地分类了396个.
鉴定出22个以前未经表征的俄罗斯,白俄罗斯和波兰的分离物属于哈勒姆基因型,由WGS证实.
突出了32个孤立物体中与spoligotyping的差异,强调了传统方法的局限性.

结论:

开发的RT-PCR试验提供了一个可靠和高效的工具,用于检测哈勒姆基因型在前性和前性研究.
这些发现表明,哈勒姆基因型在全球某些地区的流行率被低估了.
强调在解释具有显著间距删除的spoligoprofiles时需要谨慎.

关键词:

哈勒姆的基因型哈勒姆的基因型结核病菌菌菌的结核病菌的结核病菌.在Spoligotyping中,使用的是Spoligotyping.整个基因组测序的测序

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