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斯特鲁维特沉背后的微生物因素:酶和细胞外聚合物质 (EPS) 作为关键参与者

Pelina Toprak¹, Anant Aishwarya Dubey¹, Abhijit Mukherjee¹

¹School of Civil and Mechanical Engineering, Curtin University, Perth, Australia.

Applied microbiology and biotechnology

|December 11, 2025

概括

微生物酶和细胞外聚合物物质 (EPS) 显著影响斯特鲁维特晶体的形成和形态. 适度的酶活性和EPS生产促进了最佳的 struvite 沉,影响其在生物技术应用中的使用.

科学领域:

微生物学微生物学
地质化学地质化学
材料科学材料科学材料科学

背景情况:

斯特鲁维特沉受各种环境中的微生物代谢活动的影响.
微生物酶和细胞外聚合物 (EPS) 在 struvite 形成中的特定作用尚未完全理解.

研究的目的:

研究微生物特征,包括尿酶,性酸酶活性和EPS,对 struvite 沉的影响.
分析不同微生物物种 (Sporosarcina pasteurii, Bacillus subtilis, Pseudomonas fluorescens) 对 struvite 矿化和形态学的影响.

主要方法:

研究了三种不同的细菌培养物介导的 struvite 沉动力学.
采用了微观结构和矿物学分析,包括场辐射扫描电子显微镜,共聚焦激光扫描显微镜,X射线衍射和里埃变换红外光谱学.
评估了细菌的尿解和性酸酶活性,EPS生产和生物膜形成.

主要成果:

适度的尿酶和酸酶活动 (77.5 U/mL) 与适度的EPS (~6.5 g/L) 产生了最大数量的斯特鲁维特晶体 (~103 μm).
较高的尿酶活性 (8.4 U/mL) 与较低的EPS (2 g/L) 导致较大的斯特鲁维特晶体 (~180 μm).
适度的生物膜形成 (OD570~1.2) 最大化了 struvite 沉,表明它在核和积累中的作用.

结论:

酶活性和EPS表现出一种复杂的相互作用,塑造了斯特鲁维特晶体结构和特征.
微生物物种,酶和EPS显著影响生物质 struvite 的结晶性和沉模式.
研究结果为设计结构生物的定制生物技术应用提供了洞察力.

关键词:

细菌-生物膜-生物矿物相互作用聚焦激光扫描显微镜 (CLSM) 的使用细胞外聚合物质 (EPS) 是一种微生物诱导的斯特鲁维特沉 (MISP)微生物介导的斯特鲁维特.

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