压力增强了大鼠海马体中结合蛋白和NMDAR亚单元基因的表达
Aravind Parthasarathy1, Ramesha Hanumanthappa1, Sarojini R Bulbule1
1Neurochemistry Lab, Department of Biochemistry Karnatak University Dharwad India.
概括
在老鼠中,由化学物质或不移动引起的压力增加了结合蛋白和海马体中N-甲基-d-酸受体子单元的表达. 这与类似抑郁症的行为和海马神经元失调相关.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 压力研究 压力研究
背景情况:
- 氧化应激会破坏信号传递和结合蛋白的功能.
- 在体内研究表明,低水平的压力可以在老鼠中诱导类似抑郁的行为.
- 之前的研究在体外压力模型中探索了基因过度表达.
研究的目的:
- 为了研究压力下的大鼠海马体中关键结合蛋白和NMDA受体子单元的基因表达.
- 为了将分子变化与海马体的行为和结构变化相关联.
主要方法:
- 威斯塔尔大鼠在30天内暴露在化学 (H2O2) 或物理 (固定) 压力下.
- 在海马体中分析了氨酸,卡尔莫杜林,氨酸,synaptotagmin,calreticulin和NMDA受体子单元 (GluN1,GluN2A,GluN2B) 的基因表达.
- 评估了抗氧化剂测定,海马神经元结构和类似抑郁的行为.
主要成果:
- 与对照组相比,在两个压力组中,氨酸,氨酸,synaptotagmin和NMDA受体亚单元GluN1的基因表达显著上调.
- 在CA2区域的海马神经元在压力较大的老鼠中显示出较少的数量和无组织.
- 受到压力的老鼠表现出运动能力低下和较低的糖糖偏好,表明类似抑郁症的行为.
结论:
- 无论是化学还是物理压力,都会改变大鼠海马体中的结合蛋白和NMDA受体基因表达.
- 这些分子变化与海马神经元的结构损伤和类似抑郁的行为有关.
- 低水平的活性氧和物种 (RONS) 可以诱导压力,影响神经元信号传递,导致抑郁症状.
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