全基因组测序作为Salmonella血型定型的可靠替代方案:与黄金标准方法进行比较研究
Irene Mileto1,2, Greta Romano1, Stefano Gaiarsa1
1Department of Microbiology and Virology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Frontiers in microbiology
|December 12, 2025
概括
全基因组测序 (WGS) 提供了一种可靠的方法来分类Salmonella enterica血清型,其性能优于传统方法. 这种方法可以准确地识别血清型,并可以解决黄金标准方法失败的情况,改善食品传播病原体监测.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 食品安全 食品安全
背景情况:
- 沙门氏菌 (Salmonella enterica) 是一种重要的食源性病原体,引起了全球健康问题的关注.
- 基于O和H抗原的血型鉴定对于沙门氏菌的分类至关重要,但很费力.
- 传统方法在分类2600多种沙门氏菌血清型时面临着速度和准确性的挑战.
研究的目的:
- 评估全基因组测序 (WGS) 作为沙门氏菌 (Salmonella enterica) 的分类方法.
- 为了将基于WGS的血清分型与黄金标准血清凝结方法进行比较.
- 评估WGS用于识别沙门氏菌血清型的效率和准确性.
主要方法:
- 从2021年1月到2024年12月收集了282个单独的沙门氏菌.
- 通过血清凝结,使用O和H抗原进行黄金标准血清定型.
- 使用Illumina MiSeq进行全基因组测序 (WGS),并使用SeqSero2和SISTR工具分析读数.
主要成果:
- 在282个分离物中,WGS准确地预测了236个分离物中的血清型,与黄金标准方法具有很高的一致性.
- 基因组类型成功识别了34个隔离物,而金标准方法失败了.
- 工作组分类系统对所有分离物进行了分类,并与现有的沙门氏菌亚种和血清菌分类保持一致.
结论:
- 全基因组测序是一种强大的,可靠的方法来确定沙门氏菌的类型.
- 与传统方法相比,WGS在速度和样本吞吐量方面具有优势.
- WGS增强了识别能力,成功地类型化了对黄金标准方法具有挑战性的隔离物.
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