一种高吞吐量检测法,用于检测酶性聚乙烯氧化
Ross R Klauer1,2, Mekhi Williams1, Darien K Nguyen1,2
1Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716.
概括
研究人员开发了一种新的高通量屏幕来检测塑料氧化,有助于发现用于回收非PET塑料的酶,如低密度聚乙烯 (LDPE). 这种方法加速了对塑料废物的生物解决方案的发现.
科学领域:
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
- 环境科学 环境科学
背景情况:
- 生物塑料回收利用为传统方法提供了可持续的替代方案.
- 虽然聚乙烯二甲 (PET) 的回收正在推进,但其他塑料的酶,如低密度聚乙烯 (LDPE),是稀缺的.
- 低低压塑料塑料 (LDPE) 占塑料垃圾的很大一部分,需要新的分解策略.
研究的目的:
- 开发一种高通量选方法,用于识别能够分解非PET塑料的酶.
- 检测聚合物中C-H键与化物的初始氧化.
- 为了加快发现聚烯分解酶的发现.
主要方法:
- 使用4-hydrazino-7-nitro-2,1,3-benxoxadiozole hydrazine (NBD-H) 开发了一种新型的高通量屏幕,用于检测聚合物氧化过程中形成的化物.
- 探针与化物反应,在等离子体氧化LDPE薄膜上形成光.
- 福里埃变换红外光谱法 (FTIR) 和接收器运行特征 (ROC) 分析被用来验证试验的有效性.
主要成果:
- 化生成与FTIR检测到的化峰值有很强的相关性 (R2 = 0.92).
- NBD-H探测器成功地确定了LDPE活性染料脱色过氧化酶 (DyPs),这些染料在LDPE薄膜上产生化物 (1.73.0折变化).
- 该试验显示出高可靠性,ROC面积在0.95.5的曲线下.
结论:
- 开发的试验为选LDPE氧化提供了一个有效的平台.
- 这种方法可以并行和自动化,以加快发现聚烯分解酶的速度.
- 这些发现为推进非PET塑料的生物循环利用铺平了道路.
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