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Ádám Kerek^1,2, Bence Török¹, Ábel Szabó¹

¹Department of Pharmacology and Toxicology, University of Veterinary Medicine Budapest, Budapest, Hungary.

Frontiers in veterinary science

|December 12, 2025

概括

抗菌素耐药性 (AMR) 是一个日益增长的威胁. 对大肠杆菌中潜在的硫胺的暴露会导致显著的遗传变化,导致广泛的交叉耐药性,并强调需要谨慎使用抗微生物药物.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
进化生物学进化生物学

背景情况:

抗菌素耐药性 (AMR) 是一个关键的全球健康挑战.
强化硫胺酸在人类和兽医中经常使用.
了解耐药性机制对于对抗AMR至关重要.

研究的目的:

为了研究大肠杆菌对强化硫胺的度增加的体外适应.
确定这种适应和潜在的耐药性共同选择的基因机制.
评估MEGA板块进化模型在研究耐药性发展中的有效性.

主要方法:

利用MEGA板的进化模型,将大肠杆菌ATCC 25922暴露在增强硫胺度的梯度 (0x到1000x) 中.
从不同度区域分离和分析克隆.
使用最小抑制度 (MIC) 测试确定表型耐药性.
通过下一代测序进行基因型分析以确定突变.

主要成果:

适应高硫胺度 (1000倍) 导致多种抗生素的MIC值显著增加.
在关键叶酸通路基因 (folP,folA) 和排泄调节基因 (emrR,marR,acrR,mdtM) 中发现突变.
观察到多种多重药物排放系统 (acrAB-tolC,emrAB-tolC,mdtEF-tolC) 和参与SOS反应调节的基因 (recN,recQ, uvrB) 的激活.

结论:

在实验室中,微观进化适应潜在的硫胺酸会诱导大肠杆菌的广泛遗传变化.
这些适应可以通过共同选择驱动交叉耐药性,影响多种抗生素类别.
这些发现强调了明智使用组合抗微生物药物的重要性,以防止抗菌素耐药性反应.

关键词:

抗菌药物反症 (AMR) 是一种埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.这就是MEGA板块.国家储备系统 (NGS)共同选择共同选择微观进化的微观进化有潜力的硫胺基.耐药性的发展耐药性的发展

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