RNA结合蛋白PRRC2B在饥饿期间保留5'TOP mRNA,以在营养恢复期间维持核糖体生物发生
Nadav Goldberg1, Doron Bril1, Miriam Eisenstein1
1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
Nucleic acids research
|December 12, 2025
概括
蛋白质PRRC2B通过与5'TOPmRNAs结合来调节代谢转变期间的核糖体生物发生. 它的缺失阻碍了营养恢复后的核糖体蛋白转化.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- PRRC2B是一种内在无序的RNA结合蛋白,参与翻译.
- 替代拼接生成了不同的PRRC2B长和短异型.
- 这两种异构体都与40S核糖体子单元和翻译启动因子相互作用.
研究的目的:
- 研究PRRC2B异型的功能,特别是长异型.
- 确定PRRC2B的新型交互伙伴和功能.
- 了解PRRC2B在细胞对代谢压力的反应中的作用.
主要方法:
- 基于质谱的相互作用研究.
- 对替代拼接的mRNA转录的分析.
- 使用5'TOPmRNA进行RNA结合试验.
- 研究PRRC2B在氨基酸饥饿和营养恢复过程中的作用.
主要成果:
- PRRC2B长异型与LARP1相互作用,并与5'TOP mRNAs结合.
- 在饥饿期间,PRRC2B对于维持核糖体蛋白的mRNA水平至关重要.
- 缺少PRRC2B会影响营养恢复时的核糖体蛋白转化.
- PRRC2B调节核糖体生物发生和翻译启动.
结论:
- 作为对代谢变化的反应,PRRC2B在调节核糖体生物发生过程中发挥着至关重要的作用.
- PRRC2B的长异构体在保护蛋白质合成至关重要的mRNA水平方面具有新的功能.
- 在代谢压力期间,PRRC2B充当关键调节者,将翻译和核糖体生物发生与核糖体生物发生联系起来.
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